You obtain the following results for the transport of an amino acid into bacteria. Based on this (make a sketch of data, does transport of diffusion (mediated)? Explain your reasoning a rate vs concentration graph to help answer this question. Initial Uptake Rate Concentration (microM) outside of (Flux) (arbitrary units) cell 110 1 220 2 480 5 830 10 1700 30 2600 100 3100 500 b) What additional experiment(s) would you perform to verify that a transport protein is or is not involved?

uring dialysis, a semi-permeable membrane with a molecular weight cutoff of 10,000 is used. Select the true statements from the list below (more than one answer could apply) Group of answer choices A monoclonal antibody with a 150 kDa MW is retained and does not diffuse across the membrane A small enzyme with 20 kDa MW diffuses readily across the membrane Buffer salts with molecular weights ranging between 100 and 500 Da diffuse across the membrane until equilibrium (equal concentration) is reached on both sides of the membrane None of the above statements are correct Please answer asap

List two differences you would expect to see in the composition of lipids in the E. coli membrane when the cells are incubated at 25 °C compared to incubation at 37 °C.

The studies of Palade and colleagues used pulse chase labeling with radioactively labeled amino acids and auto radiography to visualize the location of newly synthesized proteins in pancreatic acinar cells. These early experiments provided invaluable information on protein synthesis and intercompartmental transport. New methods have replaced these early approaches, but two basic requirements are still necessary for any assay to study this type of protein transport. What are they, and how do recent experimental approaches meet these criteria?

The classic demonstration that cell plasma membranes are composed ofbilayers depends on the following kinds of data:• The membrane lipids from 4.74 x 109erythrocytes will form a monolayer of area 0.89 m2when spread on a water surface.• The surface of one erythrocyte is approximately 100 μm2in area. Show that these data can be accounted for only if the erythrocyte membrane is a bilayer.

Describes a process that can be optimized with the incorporation of a membrane bioreactor

One question with 4 parts

A cell with a K+1 concentration of 0.3 eq/L is placed into a solution with a K+1 concentration of 0.2 eq/L.  It is assumed that the K+1 can pass through the cell membrane (the K+1 transport protein is open). Answer the following true or false questions. 1. K+1 will diffuse through the membrane down its concentration gradient due to Kinetic Theory  2. Movement of a solute through the membrane is called dialysis 3. K+1 will move out of the cell

Various chemical methods can be used to permeabilize or lyse cells including the use of enzymes. List three important factors to consider when developing a lysis method using enzymes.   Estimate the osmotic pressure drop across the membrane of an animal cell in a 0.10 M NaCl solution, assuming that the internal total solute concentration is 0.36 osM and temperature is 30.0°C. Do you think that this pressure drop would lyse cells? Why or why not?