You isolate ten new mutant yeast strains that are defective in synthesis of leucine, an amino acid. These Leu- mutants (numbered 1-10) were all isolated in a strain of mating type a (MAT a). S. cerevisiae yeast are either mating type a or α. As it turns out, your high school classmate, now at the University of Chicago, has independently isolated ten yeast Leu- mutants (numbered 11-20) in a strain of mating type α (MAT α). You and your ex-classmate decide to combine your resources and determine how many different genes are represented by your 20 mutant strains. You cross each of the MAT a strains to each of the MAT α strains. Your experimental observations are shown in the table below, where an empty square indicates that the diploid did not grow on minimal medium and a filled square indicates that the diploid did grow on minimal medium. Based on this experiment, what is the minimum number of genes required for leucine synthesis?
5. You isolate ten new mutant yeast strains that are defective in synthesis of leucine, an amino
acid. These Leu- mutants (numbered 1-10) were all isolated in a strain of mating type a (MAT a).
S. cerevisiae yeast are either mating type a or α. As it turns out, your high school classmate,
now at the University of Chicago, has independently isolated ten yeast Leu- mutants (numbered
11-20) in a strain of mating type α (MAT α). You and your ex-classmate decide to combine your
You cross each of the MAT a strains to each of the MAT α strains. Your experimental
observations are shown in the table below, where an empty square indicates that the diploid did
not grow on minimal medium and a filled square indicates that the diploid did grow on minimal
medium.
Based on this experiment, what is the minimum number of genes required for leucine
synthesis?
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