Methyl iodide (CH31) can be used to modify DNA in solution. It takes the following mechanism for this reaction: Step 1. (slow) CHз снз* + г Step 2. (fast) DNA + CH3* > DNA-CH3* Which of the following is true about this reaction and its mechanism? O a. lodide ion (I ") is an intermediate in the reaction. Ob.CH3* is a catalyst of this reaction. O. The activation energy of Step 1 must be lower than the activation energy of Step 2. d. The overall reaction order of the reaction predicted by this mechanism is 1st order. By increasing temperature, the rate constant of Step 1 will increase but the rate constant of Step 2 will е. decrease.

Considering PNPP → PNP reaction, would you expect to see more intense or pale color for the reaction that contain the inhibitor? Explain.

5) In the below two step transformation, the first step AG is positive i.e. 1.7 kJ/mol. Yet this two step coupled reaction takes place to form Fructose-1.6-bisphosphate. Explain why?" C-H CH-OH CH,-0 H-C-OH AG"- 1.7 KJ/mol (+0.4 kcalmol) C=0 AG"=-14.2 k/mol(-3.4 kcalmol) HO-C-H HO-C-H HO-C-H H-C-OH H-C-OH H-C-OH H-C-OH H-C-OH ATP ADP H-C-OH CH-0-P. CH,-0-P-o- CH,-o- Glucose-6-phosphate Fructose-6-phosphate Fructose-1,6-bisphosphate

The catalytic triad consists of Asp, His, and Ser. What would be an alternative for histidine and how would we draw the interactions in a mechanism?

Define the following:  1. Cofactor 2. Coenzyme 3. "-ase" ending

Indicate which of the following descriptions is true about the biochemical depicted here: NH₂ +41 5 HO- он он OH OH OH a. Ribonucleotide b. Complementary to dTTP c. Purine d. Key reagent in Sanger sequencing e. Answers b., c. and d. are correct

Can you please help show how to find Kr?

what is meant by S195? D102? H57? What do you figure would happen to the enzymes catalytic activity if the amino acids above were mutated?

Match the following nucleobases (designated AD) with their names and answer questions about them. NH, NH, C D NH2 Note that some of the items from the answer list should NOT be used. Guanine 1. Aand B - Cytosine 2 Cand D • Adenine 3. Not shuwn v Thymine 4. D v Uracil 5. B a Aand C v Which of tho shown bases are pyrimidines? 7. A and O v Which of the shown bases form canonical A-T Watson-Crick base pair? S. A v Which of the shown bases form canonical G-C Watson.Crick base-pair? 9. Band C 10. C 11. Band D