You and a partner conduct the acid-fast staining using the same two bacteria. You know that there will be an acid-fast bacteria present. Your slide shows acid-fast and non-acid fast bacteria. Your partners show both bacteria are non-acid fast. Your partner doesn’t understand why. What errors do you think occurred? Please state 2 and explain why these errors would cause this result.

Select all that apply to a negative stain:    1. involves a washing step 2. cells may be distorted or shrunken  3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells

For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).             Stain Cationic or Anionic Dye malachite green   crystal violet   nigrosin   safranin

You can see your cells using the scanning and the 10x objective lenses. You cannot focus on anything when you add oil to your slide and move to the oil immersion objective. Your instructor comes over and wipes off your oil, turns over the slide, adds new oil, and moves the oil immersion objective into place. You can now focus on your cells. Knowing how immersion works, why couldn't you see the cells when the slide was upside down?

The GRAM stain method was applied on both pictures. What is the result found on each of them? A B 宁 键

Which of the following technique is FALSE in microscopy? Osmium tetroxide can be used as a fixative as well as a negative stain in transmission electron microscopy. Two proteins; each tagged with their individual primary antibody followed by one protein with rhodamine conjugated secondary antibody and the other with fluorescein-conjugated secondary antibody can be visualized simultaneously using a fluorescence microscope. The emission spectrum of Rhodamine is 580 nm The emission spectrum of Fluorescein is 521 nm O An objective lens with 100X magnification and projection lens with 10X magnification is going to produce a 1000X total magnification. Phase contrast and differential interference contrast (DIC) do not require staining.