You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%

There are eighteen (18) rows of boxes, for around 36-38 boxes total. Please see images attached. This is a pathway trace, with the final box showing "ribose 5 phosphate" as the end product.    Any help is appreciated-thank you!    glutamate to ribose 5 phosphate  No a ketoglutarate dehydrogenase  No pyruvate carboxylase  No transketolase  No transaminase  You do get to have if needed: erythrose 4 phosphate asparate dihydroxyacetone phosphate bicarbonate ATP ornithine CO2

How many ATP are produced from the complete degradation of 1,3-bisphosphoglycerate? the complete degradation of ONE glucose using the malate-aspartate shuttle as your guide.

Define a ketogenic diet and give an example of a ketogenic meal. Explain why ketone supplements would not be a useful alternative to the ketogenic diet as a cancer therapy. Why might dietary treatments be preferred over conventional treatment options?