Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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The scientist then loads a gel with a pH gradient and
separates the same set of proteins with isolectric focusing.
Arrange the proteins by expected order of migration from the
cathode to the anode.
Cathode (-)
Anode (+)
pyruvate kinase
Answer Bank
phosphoenolpyruvate carboxylase
ornithine decarboxylase
lactate dehydrogenase
cytochrome c
aldolase
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Transcribed Image Text:The scientist then loads a gel with a pH gradient and separates the same set of proteins with isolectric focusing. Arrange the proteins by expected order of migration from the cathode to the anode. Cathode (-) Anode (+) pyruvate kinase Answer Bank phosphoenolpyruvate carboxylase ornithine decarboxylase lactate dehydrogenase cytochrome c aldolase
The isoelectric points and molecular weights for a set of proteins are given in the table.
Protein
aldolase
cytochrome c
pyruvate kinase
lactate dehydrogenase
ornithine decarboxylase
phosphoenolpyruvate carboxylase
Isoelectric point, pl Molecular weight (kDa)
40
14
62
18
75
74
10.1
9.2
6.0
8.5
4.1
5.0
A scientist loads the proteins into the top of a sodium dodecyl
sulfate (SDS)-polyacrylamide gel and subjects them to
electrophoresis. Researchers refer to this technique as SDS-
PAGE. Arrange the proteins by expected order of migration
in an SDS-PAGE gel from the top of the gel to the bottom.
Top of the gel
Bottom of the gel
Answer Bank
ornithine decarboxylase
cytochrome c
pyruvate kinase
phosphoenolpyruvate carboxylase aldolase
lactate dehydrogenase
expand button
Transcribed Image Text:The isoelectric points and molecular weights for a set of proteins are given in the table. Protein aldolase cytochrome c pyruvate kinase lactate dehydrogenase ornithine decarboxylase phosphoenolpyruvate carboxylase Isoelectric point, pl Molecular weight (kDa) 40 14 62 18 75 74 10.1 9.2 6.0 8.5 4.1 5.0 A scientist loads the proteins into the top of a sodium dodecyl sulfate (SDS)-polyacrylamide gel and subjects them to electrophoresis. Researchers refer to this technique as SDS- PAGE. Arrange the proteins by expected order of migration in an SDS-PAGE gel from the top of the gel to the bottom. Top of the gel Bottom of the gel Answer Bank ornithine decarboxylase cytochrome c pyruvate kinase phosphoenolpyruvate carboxylase aldolase lactate dehydrogenase
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