you have a cell that carries mutations in the lac operon repressor gene that render it unable to bind the operator, but otherwise has a fully funcitonal lac operon. you introduce a plasmid carrying a funcitonal lac repressor gene into these mutant cells.  Which best describes the likely outcome, in the absence of lactose

select all the mutations that could apply

You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan.  Using your knowledge of operons and their regulation, answer the following questions  I really need a in depth epxplantion for the queshtions to make sure i understand the answers    a.What effect does lactose have on the bacterial cell’s lac operon?    b.What does the absence of glucose do to the bacterial cell?    c.You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon?   d.What kind of regulation does lactose provide to an operon’s repressor?

You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan.  Using your knowledge of operons and their regulation   a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?

Bob determines that Red/pink protein is synthesized at much higher amounts than the Brown protein; Bob thinks that this is how the burger always is perfectly medium-rare (pink/red in the interior of the patty, with a small amount of brown at the burger’s surface). Bob also knows that R and B both are transcribed from the single operon. Briefly describe the biological mechanism allowing for accumulation of more R than B protein.

Suppose that  a  new  mutation  lacIes, ('es'  stands  for  ‘extra-strength’)  has been discovered in the  lac operon  that allows  the  lac-repressor  to bind even  to  the  lacOc  operator.  Other  properties of  lacIes repressor  remain  normal (that is the same  as  of  lacI+).  Given  this genotype    Is P+ Oc Z+ Y-  / Ies  P+  O+ Z+  Y+  ,  under  which conditions will the gene  lacZ  be  transcribed  to  produce  β-galactosidase and the gene  lacY  transcribed to  produce  permease?     A)  With  lactose,  β-galactosidase and permease are  produced, without  lactose they  are  not produced B)  With  lactose,  β-galactosidase is produced, without  lactose it is not;  permease is never produced C)  β-galactosidase is always produced  and  permease is never  produced D)  With  lactose,  β-galactosidase and permease are  produced, without  lactose they  are  also produced E)  β-galactosidase is always produced  and  permease is produced only  in the  presence of  lactose

There is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers.   a-Lactose present, glucose present b-Lactose present, glucose absent   c-Lactose absent, glucose present   d-Lactose absent, glucose absent