You are trying to determine the cell count per microliter of some melanoma cells that have been cultured and are going to be used for isolating RNA. The RNA kit says that you need no more than 2.5 x 106 cells to use for the isolation protocol. When you lift and count from your cell culture plate, you find 253 viable cells in 5 of of the small grid squares (1/25 sq mm) of your hemocytometer. How many cells/ml does your sample contain? What volume will you use for the RNA isolation? Group of answer choices 1.012 x 10 to the 3rd cells/ul; 40 ul 1.265 x 10 to the 7th cells/ul; 5.06 ul not possible to answer with the information provided 1.265 x 10 to the 6th cells/ul 0.197 ul

You are trying to determine the cell count per microliter of some melanoma cells that have been cultured and are going to be used for isolating RNA. The RNA kit says that you need no more than 2.5 x 106 cells to use for the isolation protocol. When you lift and count from your cell culture plate, you find 253 viable cells in 5 of of the small grid squares (1/25 sq mm) of your hemocytometer. How many cells/ml does your sample contain? What volume will you use for the RNA isolation? Group of answer choices 1.012 x 10 to the 3rd cells/ul; 40 ul 1.265 x 10 to the 7th cells/ul; 5.06 ul not possible to answer with the information provided 1.265 x 10 to the 6th cells/ul 0.197 ul

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A amp PBR322 4301 fot B Clear Zones Figure 2 The postgraduate student, Demika, inserted her gene of interest into the plasmid, pBR322, before transformation into the competent host cell using heat shock method. After that she cultured the cells on the Ampicillin agar plate before replica plating the colonies onto another Ampicillin (A) and Tetracycline (B) agar plates shown in Figure 2. (1) Referring to the vector pBR322 in Figure 2, which recognition site was cleaved to insert the gene of interest? Based on the observation above, can you identify which colonies are carrying positive mcombinants of BR322? Explain your selection.
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If you extract the DNA of the coliphage φ X174, you willfind that its composition is 25 percent A, 33 percent T,24 percent G, and 18 percent C. Does this compositionmake sense in regard to Chargaff’s rules? How wouldyou interpret this result? How might such a phage replicate its DNA
5 μL of plasmid DNA (pUC 19) was added to 50ul of CaCl2 competent E. coli that was then added 250 μL of SOC medium, 100 μL of this solution was plated onto a TSA + ampicillin plate. Can you please show me how I calculate the total number of successful transformants per mL of competent cells plated. The total number of colnies counted on plate was 70. Thank you
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you have transformed E.coli cells with a plasmid containing the gene for the enzyme beta-lactamase you have used 3µl of a stock solution of 2.5ng/µl DNA. You transformed 150/µl of competent cells, using the calcium chloride method. you have added 600µl of luria broth to the cells and then plated out 200µl on triplicate plates. upon counting the colonies, you got the following results: plate 1: 120 cfu plate 2: 35 cfu plate 3: 95 cfu please answer the following question: 1. what new characteristic will the cells have ater transformation? 2. how are you going to test for the new characteristic? 3. calculate the transformation efficiency of the experiment?
You are about to isolate a 3000 bp large plasmid from an E.coli culture. You know that the plasmid is present in 100 copies per E. coli cell. You aim to have a final plasmid concentration of 100 ng/µl in a total volume of 50 ul. Assuming the yield is 100 %, how many E. coli cells should the culture from which the plasmid is to be isolated at least contain A) 4.7 · 1013 cells B) 1.6 · 1010 cells C) 3.2 · 1010 cells D) 3.2 · 1026 cells E) 1.6 · 1012 cells Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable. Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments migrate faster than larger fragments because they can move faster through…