The schematic on the right is for which molecular biology method?             What information does this method reveal?

What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 2 reasons.

What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein) occurred in those cells in which CRISPR worked?

Your colleague wants to align the complete sequence of the collagen gene from humans to the homologous complete gene in rats. She can't decide if she should use the alignment program Needle or Water, and turns to you for help. Does it make a difference which program she uses, what advice would you give her?

After running a qPCR experiment, we will have graphs showing the amount of fluorescence detected by the digital camera compared to the number of PCR cycles run. Suppose you see the following graph output by the qPCR machine: Relative Fluoresence 3.0 2.5 2.0 1.5 1.0 0.5 0.0 0 10 20 30 40 50 Cycles Which curve (blue, red, or green) represents a sample with the smallest amount of mRNA present? Why? Be sure to discuss Ct values in your answer.

Select all that would be true if I had a nonsense mutation in an exon of a gene: The nonsense mutant allele would be the same size as wildtype by PCR-electrophoresis The nonsense mutant protein would be the same size by Western as the wildtype protein The nonsense mutant allele would be a different size compared to wildtype by PCR- electrophoresis The nonsense mutant protein would be a different size by Western compared to the wildtype protein

What is the program Finch TV used for?

Which of the following technologies would be useful to assess natural patterns of mRNA abundance in the brain of a reptile species that has never been the focus of genetic research before? Group of answer choices DNA microarray RNA-sequencing with Illumina DNA-sequencing with Illumina RNA microarray

What is the Lineweaver-Burk plot and what does it tell us?

What are some of the technical challenges of cloning a mammoth?  Check all that are true   Ancient mammoth DNA has degraded so it is hard to know the complete genome sequence   Mammoth gestation time is likely too short to allowing cloning   A living surrogate mother would have to be used, which could pose problems since the closest relative to mammoths is endangered elephants   A living oocyte would have to be obtained from an extant species, which could pose problems since the closest relative to mammoths is endangered elephants   Mammoths lived so long ago that they used a different genetic code than modern animals   There are no enzymes in existence that could ligate together mammoth DNA sequence with elephant sequence   Mammoths probably had egg shells, which would be hard to penetrate with a needle