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The schematic on the right is for which molecular biology method?             What information does this method reveal?

The schematic on the right is for which molecular biology method?             What information does this method reveal?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The schematic on the right is for which molecular biology method?  

 

 

 

 

 

What information does this method reveal? 

 

**Transcription and Explanation for an Educational Website** **Title: Understanding Gel Electrophoresis in Protein-DNA Interactions** **Diagram Explanation:** The image illustrates the process of gel electrophoresis used to study protein-DNA interactions, particularly focusing on the binding of transcription factors (TF) to DNA probes. The diagram is divided into two main parts: **A: Mixture Preparation** - This section shows a test tube containing a mixture of DNA probes and transcription factors (TF). - The DNA probes are represented as elongated rectangles, some of which have transcription factors bound to them. - The free probes are depicted as having no transcription factor attached. **B: Gel Electrophoresis Process** - The polyacrylamide gel is used to separate different complexes based on size and charge. - The gel contains several wells (topmost) where the prepared samples are loaded. - As the samples migrate through the gel: 1. **Complex Supershifted By Antibody:** - These are shown as the slowest migrating bands in the gel. - They consist of DNA probes bound to transcription factors, which are further bound by specific antibodies, increasing the molecular weight and causing a supershift. 2. **Protein Bound Probe:** - This section shows DNA probes bound to transcription factors. - These complexes migrate more slowly than unbound probes but faster than complex supershifted by antibody. 3. **Unbound Probe:** - Represented at the bottom, these probes migrate the fastest through the gel. - They indicate the portion of DNA probes that are not bound by any transcription factor. **Conclusion:** This diagram provides a visual representation of how gel electrophoresis can be used to analyze the binding interactions between proteins (such as transcription factors) and DNA. By comparing the migration patterns of different complexes, researchers can infer the presence and strength of protein-DNA interactions.
Transcribed Image Text:**Transcription and Explanation for an Educational Website** **Title: Understanding Gel Electrophoresis in Protein-DNA Interactions** **Diagram Explanation:** The image illustrates the process of gel electrophoresis used to study protein-DNA interactions, particularly focusing on the binding of transcription factors (TF) to DNA probes. The diagram is divided into two main parts: **A: Mixture Preparation** - This section shows a test tube containing a mixture of DNA probes and transcription factors (TF). - The DNA probes are represented as elongated rectangles, some of which have transcription factors bound to them. - The free probes are depicted as having no transcription factor attached. **B: Gel Electrophoresis Process** - The polyacrylamide gel is used to separate different complexes based on size and charge. - The gel contains several wells (topmost) where the prepared samples are loaded. - As the samples migrate through the gel: 1. **Complex Supershifted By Antibody:** - These are shown as the slowest migrating bands in the gel. - They consist of DNA probes bound to transcription factors, which are further bound by specific antibodies, increasing the molecular weight and causing a supershift. 2. **Protein Bound Probe:** - This section shows DNA probes bound to transcription factors. - These complexes migrate more slowly than unbound probes but faster than complex supershifted by antibody. 3. **Unbound Probe:** - Represented at the bottom, these probes migrate the fastest through the gel. - They indicate the portion of DNA probes that are not bound by any transcription factor. **Conclusion:** This diagram provides a visual representation of how gel electrophoresis can be used to analyze the binding interactions between proteins (such as transcription factors) and DNA. By comparing the migration patterns of different complexes, researchers can infer the presence and strength of protein-DNA interactions.
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