#6 help You are a scientist studying the function of a gene you find interesting. You need to produce protein in E.coli so you can purify it andrun for functional assay. Which of the following steps is not part of the process of cloning the gene?Amplifying the target gene through PCR.Cutting both foreign DNA and plasmid DNA with the same restriction enzyme.Adding DNA ligase to create a hydrogen bond to seal the nick in the backbone.Introducing circular recombinant DNA to host bacterial cells.Selecting the cells with recombinant DNA using selectable markers.goo

The synthesis of proteins is essential to biological processes and has numerous applications in…

Answered: You are a scientist studying the…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 8QP

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Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Which of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Which of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmid
A double-stranded length of DNA is exposed to a restriction enzyme. The enzyme finds 3 recognition sites. How many fragments will be produced? a) 2 b) 3 c) 4 d) 5
Label the image below with ALL the pertinent information related to gene cloning. Make sure you use the following terms: bacterial plasmid, the gene of interest, recombinant plasmid, restriction enzymes, DNA ligase, insert plasmid into bacteria, cloning of plasmid in culture, etc.)
Which of the following would you need to clone a human gene? Choose all that apply A restriction enzyme Human DNA A donor goat nucleus An enucleated egg A surrogate mother DNA ligase A plasmid vector (or other type of DNA vector, e.g. phage, BAC)
You’re working in a research lab, and your current task is to clone the gene that codes for tyrosinase from potatoes. You grind up some potato, extracts the DNA from it and digests the DNA with two different restriction enzymes (separately, not together): EcoRI and BamHI. You then obtain the cloning vector, pUC19, and digest it with the same two enzymes. You then run a gel which is shown to the right. You mix the potato DNA (digested with the enzyme you specified in part B) with cloning vector DNA (digested with the same enzyme). You then add the mixture to E. coli cells and carry out a transformation procedure so that the cells can each update a plasmid. You then plate the cells on a plate containing antibiotic. What antibiotic would be in the plate? Why would there be antibiotic in the plate? Be specific! Unfortunately, you don’t get a single bacterial colony to grow on the plate. Not even one! You review your procedure and realize that when mixing the digested potato DNA…
An important feature of restriction enzymes is that each enzyme only recognizes a specific palindrome and cuts the DNA only at that specific sequence of bases. A palindromic sequence can be repeated a number of times on a strand of DNA, and the specific restriction enzyme will cut all those palindromes, no matter what species the DNA comes from. A linear DNA molecule is represented below. The DNA is represented by one line, although in actuality, DNA has two strands. If the DNA molecule has two restriction sites, specifically two repeats of a specific palindrome sequence, A and B, for a specific restriction enzyme: How many fragments would be produced if the DNA is cut by that enzyme? Number each fragment Which fragment would be the largest? Which fragment would be the smallest?

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