Which of the following is NOT a tool used in recombinant DNA technology? Cutting specific DNA sequences using restriction enzymes. Adding Taq polymerase to copy DNA in PCR. Using primase to catalyze the formation of phosphodiester bonds. Inserting a gene of interest into a vector. Inserting a plasmid into bacteria to generate copies.

Choose the single most appropriate description of how most Next Generation sequencing methods work.   Template DNA is attached to 'chips', free di-nucleotides are added to synthesise new DNA, computers read the results.   Template DNA is attached to 'chips', di-deoxy nucleotides are added to synthesise new DNA, computers read the results.   Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. They are bound to a membrane and sequenced using lasers.   Sample DNA is broken into short fragments and synthetic oligonucleotides are added to them. The fragments are then bound to a membrane and sequenced using light emission as the reporter.   Sequencing by synthesis of short template fragments, using various 'reporters' and computing power to rebuild lengthy sequence data in silico using reference genomes.   Sanger sequencing of short fragments, using various 'reporters' and computing power to…

Restriction enzymes and DNA ligase are key components when preparing cloning vectors. True or False.

Describe how Restriction Enzymes, Plasmids, DNA Hybridization, and PCR can be used for molecular cloning and genetic engineering.

Which of the following components are required for the polymerase chain reaction (PCR)? Select all correct answers. Group of answer choices dNTPs a restriction enzyme DNA polymerase DNA ligase ddNTPs oligonucleotide primers

CRISPR techniques allow scientists to modify specific genes while sparing all others, thus clarifying the association between a given gene and its consequence to the organism. How can this help to create better antibiotics or to make white blood cells resistant to HIV infection? Give your examples. What is the future of CRISPR?

Discuss how the technique of PCR allows a scientist to quickly clone a particular piece of DNA.Be clear about the importance of 2 primers, Taq DNA polymerase, temp, and time. Explain howyou do a PCR – what is needed

In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C -heating the sample to 72 C -heating the sample to 95 C options to match: - allows primers to anneal to the template DNA - promotes synthesis of new DNA - separates the DNA strands in the samples

Humans have very similar DNA sequences, with approximately 999/1000 letters being ide One possible way of telling the difference between these very similar sequences is by: Genetic engineering Analyzing RFLPS using gel electrophoresis Comparing the total amount of DNA from two or more people

In the following "gene library" cloning experiment Digested genomic DNA AmpR gene TCR gene TCR is tetracycline resistant marker, AmpR is ampicillin resistant marker and BamHI is the unique restriction enzyme on plasmid. A PhD student digests/cuts the plasmids with BamHI restriction enzyme and the genomic DNA with EcoRI restriction enzyme. After performing the cloning experiment and obtaining colonies on a selection plate, the obtained cells will be ..... (Hint: this question is even more challenging; the PhD student was later demoted to an MSc student). a) resistant to ampicillin and tetracycline b) sensitive to tetracycline and ampicillin c) resistant to tetracycline and sensitive to ampicillin d) resistant to ampicillin and sensitive to tetracycline e) sensitive to ampicillin and tetracycline BamHI