List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them in the order that you would use them. Make sure to label which is which.

Q: I have a microscope with oculars that are 20x and am viewing a slide using oculars that are 50x.…

A: Introduction  An instrument used to magnify small objects called a microscope. Even at the cellular…

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: A swab for culture is received from a doctor's  office. The staff forgot to mention the source from…

A: Introduction Microbiology is the branch of science that deals with the study of different types of…

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: What are the sources of error in performing Gram staining?

A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…

Q: ext/map/ aining Lab Report Place the descriptions into the correct boxes to test your understanding…

A: Gram staining is a method frequently used to distinguish between two sizable groups of bacteria…

Q: When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the…

A: Bacteria These are prokaryotic organisms which kack membrane bound organelles. Through the technique…

Q: Name a stain (be specific) that might have been used on the slide. Os 09 5.0 μm

A: Staining is a method used to magnify and contrast a biological material at the level of the…

Q: Gram positive and Gram negative differ in their detail or diagram these differences. (two words).…

A: The gram-positive bacteria retain the crystal violet colour and stains purple whereas the…

Q: Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe…

A: 1. Glass slides on which the sample is placed. 2. Cover slip made up of plastic. 3. Dropper 4.…

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: Please write, in paragraph form, how you would identify the organism step by step. Remember to…

A: Proteus vulgaris found in people and animals' intestines are Gram-negative, rod-shaped,…

Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: Choose the 4 most correct steps from the list below for a simple positive stain and organize them…

A: The above question is related to the laboratory technique of bacterial staining. Staining is a…

Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…

A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: Table 1. Objective Lens Scanner Low Power Objective High Power Objective Oil Immersion Objective…

A: A compound microscope,  is one that uses a system of lenses and visible light to generate magnified…

Q: Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and…

A: Introduction :   Blood Agar (BA) is an enhanced medium used to culture bacteria and microorganisms…

Q: Please answer fast  What chemical is the mordant in the Gram staining procedure. Explain how it…

A: Given: Gram staining procedure was given by Hans Christian gram in 1884.It is a technique that…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: Complete the table below. For the function, identify the reagent if it is a stain, clearing agent,…

A: Answer : The function of reagents are :  1- FAA : fixative  2- ethyl alcohol : chemical solvent  3-…

Q: Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps…

A: There are several methods to differentiate bacteria into different groups, the staining method is…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: Gram staining procedure begins with preparing of a smear of bacterial culture. This  step is called…

Q: Gram staining is the most common initial test of unknown identification, explain in your own words…

A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…

Q: PREPARATION OF SMEARS: 1. Wash the slide thoroughly with a detergent, rinse with water and dry with…

A: PREPARATION OF SMEAR ( FLOWCHART)

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Which of the following can be used to distinguish one colony from another on an agar plate?  Select…

A: A colony is a visible cluster of microorganisms like fungi and bacteria that grow on a solid growth…

Q: Why is it essential that the primary stain and the counterstain be contrasting colors in…

A: The gram staining is used to distinguish between gram negative and gram positive bacteria. It was…

Q: Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps…

A: Gram staining is a staining method used to differentiate between gram-negative and gram-positive…

Q: Below I have attached the image of gram stain. -We need to find the name of the compound. And after…

A: Gram negative bacteria - The bacteria which are unable to retain crystal violet stain in the process…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…

A: Gram staining is also known as Gram's method is a staining method for identifying and separating…

Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium

A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…

Q: If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being…

A: Microscope  is an instrument used to visualize minute objects . It  consists of two types of lens…

Q: Outline the acid fast staining technique using a flow chart.

A: ACID FAST STAINING TECHNIQUE : it is the differential staining techniques which was first developed…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.