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Write a short discussion based on the graph and calculate mean growth rate and mean generation time

Write a short discussion based on the graph and calculate mean growth rate and mean generation time

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Write a short discussion based on the graph and calculate mean growth rate and mean generation time

Procedure 50 of ampicillin to the Add 3 ml Top up to 50ml. - Add fresh LB - both Mix Sml Take overnight culture mixture analyse to the mixture with 45 ml LB broth ODG00 The mixture is then Measure every 15 mins. Stop until 75 mins. Withdraw 3ml mixture to -analyse (O0600)record as zero time point. Overnight culture shaked incubated at (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture Sml Take 3 ml Add 5o ul of ampicillin to the mixture mixture to No ampicilin B (control) with 45 ml LB broth analyse OD600 the Measure every 15 mins. Stop until 75 mins. The mixture is then - incubated at Withdrew 3ml mixture to shaked analyse (OD600jrecord as zero time-point. incubator at 37c 200rpm Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) (A) (B) OD 600 - Group 3(Ampicilin) -Group 3(Control) (minutes Ampicillin Control 0.121 0.133 15 0.167 0.168 30 0.053 0.248 45 0.018 0.339 60 0.053 0.509 75 0.053 0.676 Absorbance(60onm)
Transcribed Image Text:Procedure 50 of ampicillin to the Add 3 ml Top up to 50ml. - Add fresh LB - both Mix Sml Take overnight culture mixture analyse to the mixture with 45 ml LB broth ODG00 The mixture is then Measure every 15 mins. Stop until 75 mins. Withdraw 3ml mixture to -analyse (O0600)record as zero time point. Overnight culture shaked incubated at (E-coli LB borth) incubator at 37°c 200rpm Mix overnight culture Sml Take 3 ml Add 5o ul of ampicillin to the mixture mixture to No ampicilin B (control) with 45 ml LB broth analyse OD600 the Measure every 15 mins. Stop until 75 mins. The mixture is then - incubated at Withdrew 3ml mixture to shaked analyse (OD600jrecord as zero time-point. incubator at 37c 200rpm Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) (A) (B) OD 600 - Group 3(Ampicilin) -Group 3(Control) (minutes Ampicillin Control 0.121 0.133 15 0.167 0.168 30 0.053 0.248 45 0.018 0.339 60 0.053 0.509 75 0.053 0.676 Absorbance(60onm)
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