Why would using dt Oligos to produce complimentary DNA (cDNA) from bacteria mean that your reaction will fail?

An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?

When Avery and his colleagues had obtained what was concluded to be the transforming factor from the IIIS virulent cells, they treated the fraction with proteases, RNase, and DNase, followed in each case by the assay for retention or loss of transforming ability. What were the purpose and results of these experiments? What conclusions were drawn?

In transformation, a bacterial cell takes up DNA fragments from its surroundings. The figure shows the process of natural transformation. Position the labels below with the correct images. (A) Donor and recipient genomes The donor strand is integrated into the recipient chromosome and the displaced bacterial DNA is degraded. A wild-type donor cell is in the vicinity of a double auxotroph. If the wild-type cell lyses, it releases DNA into the environment. One cell is identical to the original recipient, while the other carries the wild-type genes. A competent recipient cells a receptor site on its surface. One strand of donor DNA enters the cell and pairs with a homologous region of the bacterial chromosome The remaining DNA strand is degraded. A donor DNA strand binds to the receptor site. Reset trpC1 Wild-type donor cell Mechanism of natural transformation Donor DNA hal Receptor site- trpc/his double auxotroph Recipient cell Competent cell recipient -Bacterial chromosome (had.bpC") Ma…