Why Sanger sequencing uses ddNTPs and how they differ functionally from dNTPs. Does Sanger sequencing minimize the length of replication since phosphodiester bonds are not forned during DNA replication. Also, does it differ from dNTPs functionally since it lacks two hydroxyl groups?

Q: rovide a detailed description and hand-drawn figure for each of the following. (1) DNA…

A: DNA REPLICATION:- It is the process of making two identical daughter copies of DNA from the one…

Q: Is it biologically possible for DNA to undergo replication in vivo, without the lagging and the…

A: Introduction  When the DNA chromosome is replicated, two copies of each identical daughter cell are…

Q: An alternative to semi-conservative replication is conservative replication in which the parent…

A: DNA is a self-replicating structure that replicates in a "semi-conservative manner". DNA replication…

Q: Tick ​​the correct statements:   Remember: Tautomers are structural isomers that differ from each…

A: Ans). The first statement, the third statement, and the fourth statement are correct. Explanation:…

Q: As a result of the rotation about its six bonds, DNA can exist in a variety of forms. Determine…

A: There are 3 major DNA variants. They are A-DNA, B-DNA and Z-DNA.  In all these variants, A pairs…

Q: Which of the DNA polymerases shown in Table  have the ability to proofread?

A: The DNA polymerase enzyme uses a single stranded DNA molecule as a template and causes the synthesis…

Q: The picture below shows DNA replication Because you are reusing the old, or parent, DNA strand, DNA…

A: Since there are multiple questions and which question is to be solved has not been specified, as per…

Q: The gel pattern below was produced by the Sanger sequencing method. Determine the sequence…

A: In sanger Sequencing, the products of the nucleotides A, G, C, and T reactions are individually…

Q: To maintain uniform diameter of the DNA double helix, only purines base with each other via two…

A: DNA is the fundamental structure that carries all the information of a living thing. In most cases,…

Q: How did the results prove the semiconservative model of DNA replication? Explain.

A: The above experiment is cesium chloride density gradient centrifugation. This experiment was done by…

Q: d). It's the temperature at which the mass of single stranded DNA equals the mass of double stranded…

A: Option d is NOT a correct description of the DNA melting temperature for a system in which we have…

Q: Although DNA polymerases require both a template and a primer, the following single-stranded…

A: DNA polymerases require both a template and a primer to synthesize DNA. However, some…

Q: Sequencing of the following fragment of single-stranded DNA needs to be undertaken using the Sanger…

A: Sanger sequencing, also referred to as the “chain termination method”, may be a method for…

Q: What two basepair changes can occur when G tautomerizes to the enol form? Recall that the…

A: Introduction: Spontaneous mutations result from numerous sources such as errors during DNA…

Q: Acyclovir can be used to treat severe cases of Epstein-Barr viral (EBV) infection, but has little to…

A: Introduction:Acyclovir is an antiviral drug commonly used to treat infections caused by…

Q: As the DNA replication move toward the end of the telomeric region, leading-strand replication…

A: Telomere is the physical ends of eukaryotic chromosomes. They protects the chromosome ends from DNA…

Q: You are using these two plasmids to make a recombinant DNA molecule. You want to make a molecule,…

A: Recombinant technology includes the process in which we can introduce our gene of interest with a…

Q: During DNA replication, an incoming nucleotide is enzymatically joined to the preceding nucleotide…

A: Phosphodiester bond Explanation:-In DNA and RNA, the phosphodiester bond is the linkage between the…

Q: DNA polymerase III (the main polymerase) Helicase DNA ligase Single-stranded binding proteins DNA…

A: DNA replication is the process by which new DNA strands are produced from the old DNA strands by the…

Q: 31.) The following DNA fragment was sequenced by the Sanger method. The asterisk indicates a…

A: ANSWER;-

Q: hat role do deoxyribonucleotide triphosphates (dNTP) in DNA replication?

A: The process of replicating a double-stranded DNA molecule into 2 identical DNA molecules is known as…

Q: DNA polymerase I, DNA ligase, and topoisomerase I catalyze the formation of phosphodiester bonds.…

A: The DNA replication is a semi-conservative mechanism in which the new complementary strand is…

Q: Which of the following statements about DNA polymerase III holoenzyme from E. coli are correct. 1.…

A: DNA polymerase II  is the primary enzyme involved in DNA replication. It is a multiprotein complex…

Q: 49 N), heavy parental DNA, and all nucleotides with 14N (another isotope of N) as "nucleotide supply…

A: We can use different shades or different colors represent a daughter strand of DNA with light N…

Q: A G-C baseрair the cytosine to uracil (deamination). occurs, after two rounds of replication this…

A: Deamination is the process in which the cytosine is converted to uracil in the DNA. This results…

Q: Replication of a circular DNA molecule can occur by either theta () replication or by rolling…

A: Introduction :- Plasmids are tiny circular DNA molecules with a few to over one hundred genes. The…

Q: Does Z-DNA have major and minor grooves? Explain.

A: Answer

Q: What entropic factor destabilizes helical DNA at high temperature?

A: The double (ds) stranded DNA undergoes structural changes also known as denaturation at high…

Q: Spontaneous oxidative deamination of guanine produces the base xanthine (see figure, which also…

A: As per the Watson-Crick model of the DNA double helix:DNA is made up of two strands of…

Q: 3)(recall) Replication: what would be the CORRECT ORDER OF NUCLEOTIDES (? ?????) in the newly…

A: DNA is the deoxyribonucleic acid. It is a genetic material present in the nucleus.

Q: A solution  containing single stranded DNA with the sequence 5’ATGGTGCACCTGACTCCTGAGGAGAAGTCTNNNNN’3…

A: In biology, DNA replication is that the process of forming 2 identical replicas of deoxyribonucleic…

Q: Why does a fiber ( double stranded molecule or duplex) of DNA shorten upon drying?

A: DNA The double helical structure present inside the nucleus.

Q: What is an Okazaki fragment? In which strand of replicating DNA are Okazaki fragments found? Based…

A: DNA (Deoxyribonucleic acid) is the genetic material, which gets copied during cell division. The…

Q: If a given double stranded DNA undergoes enzymatic hydrolysis targeting only the "a" side in the…

A: The hydrolysis is targetting the 3' Carbon of the sugar in the phosphodiester bond. 1st strand…

Q: FIGURE 19.16 Direct repair of dam- aged bases in DNA. (a) The repair of thymine dimers by…

A: The DNA (deoxyribonucleic acid) is the hereditary unit. It is a double stranded structure of…

Q: Adenylate cydase, which synthesizes cyclic AMP from ATP, requires two metal ions, and the enzyme has…

A: Nucleophile is a chemical species with more electrons. This chemical species donates its electron…

Q: Cytosine

A: Introduction :- A mutation occurs when a DNA gene is damaged or changed in such a way as to alter…

Viral Replication

The virus is acellular and considered non-living outside the host cell. The replication of the virus occurs only inside the living cells. Only a few genes are present in the smallest virus, whereas many genes are present in the largest virus. These genes encode specific viral proteins.