Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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- Here’s a Ramachandran plot. Give the dihedral angles--phi and psi--that are characteristic of a left handed helix.arrow_forwardYou study actin polymerization by monitoring the increase in fluorescence of pyrene-labeled actin. Pyrene is a fluorescent molecule, which has very low level of fluorescence on its own or when fused to G-actin. However, the pyrene fluorescence increases dramatically when pyrene-labeled G-actin is incorporated into a polymer. In this assay, the amount of pyrene fluorescence is proportional to the amount of actin polymer. In the figure below, actin polymerization curves resulted for conditions listed below; assume that G-actin concentration is above the critical concentration for the pointed end and that ATP is present. Which curve corresponds to which condition? Explainarrow_forwardConsider normal B-form DNA. It forms a regular antiparallel double-helical structure with Watson-Crick base-pairing mediated through hydrogen bonding. The base pairs all stack upon one another, with 3.4 Å spacing between them. DNA strands having a complementary sequence will spontaneously form a double-helix in an aqueous solution. In terms of energy, what primarily drives helix formation? O Positive Entropy from base stacking van der Waals interactions O Hoogsteen interactions Positive Enthalpy from Hydrogen Bonding between GC and AT pairs Negative Enthalpy from Hydrogen Bonding between GC and AT pairs O Negative Entropy from base stackingarrow_forward
- Base Pairing Practice Directions: In each box, type the first letter of the base that correctly matches the given DNA sequences. C G A A C T T C C A C G C G T. m ctrarrow_forwardFrom the following DNA template, which sequence is synthesized by RNA Polymerase? 5’- T – C – C – T – A – A – G – A –3’ Group of answer choices 5’- A – G – G – A – T – T – C – T –3’ 5’- U – C – C – U – A – A – G – A –3’ 3’- A – G – G – A – U – U – C – U –5’ 3’- T – C – C – T – A – A – G – A –5’ 3’- T – G – G – A – T – T – C – T –5’arrow_forwardDNA contents of nitrogenous bases • %A = %T %C = %G • A+G = C+T %3D Example: if 35% of the bases of a DNA - molecule is thymine what the % of Cyosine?arrow_forward
- BIOCHEMISTRY. other chegg & bart answer was answered incorrectly. need first blank only Please provide only typed answer solution no handwritten solution needed allowedarrow_forwardUse 25ul for calculations.arrow_forwardOpen with Google Docs A DNA sequence is a chain of nucleotides. The nucleotides on joined together are joined the same strand are group-sugar backbone. together by the phosphate On the other hand, the nucleotides between the two strands are joined together by hydrogen bonds between the nitrogenous bases. In the box below, illustrate the DNA sequence as a chain of nucleotides with a phosphate group-sugar back bone and with hydrogen bonds between the nitrogenous bases.arrow_forward
- choices:purine or pyrimidine ribose or deoxyribose 1-9? hydrogen or phosphodiester 1-9? an -OH or no -OH 1-9?arrow_forwardWhat is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA. The Chelex denatures the DNA and makes the DNA single stranded. The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells. The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNAarrow_forwardRadioactive uracil can be used to label all the pyrimidine residues in DNA. Explain how this occurs, thinking about pyrimidine synthetic pathways.arrow_forward
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