Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli
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- Why is it necessary to perform an enrichment procedure in isolating Staphylococcus aureus instead of directly plating it into a selective medium? What might happen if you omit potassium tellurite in using GCM?What is the benefit of using LB (Lysogeny broth) medium for the growth of E. coli, Bacillus subtilis and Pseudomonas putida? Why ?you are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)
- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?Why is Micrococcus luteus a common contaminate on bacteriological media?What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or streptococcus bacteria in microbiology?
- Why is the effectiveness of an antimicrobial agent determined using an exponentially growing bacterial culture? Please explain.1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?Escherichia coli Mycobacterium phlei Bacillus Micrococcus subtilis luteus A B Figure 1: Agar Slant Cultures of Bacteria (Gary E. Kaiser, Ph.D.- Professor of Microbiology) . Observe and describe the colour of the slant cultures (A-D) in fig 1. ( . Define the following terms: pure culture, sterile medium, inoculum, aseptic technique, and colony. What is the name of the cultures that you used . Where they gram negative or positive Define the following terms: psychrophile, mesophile, thermophile, and hyperthermophile.
- E. coli O157:H7 is an organism of concern in contaminated foods. How can the use of MacConkey agar and Sorbitol-MacConkey agar be used to screen for E. coli bacteria in general and E. coli O157:H7 in particular?Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarMannitol salt agar is often used to distinguish between different species of Staphylococcus, a gram positive bacterium that is well adapted to living on dry, salty skin. Disease-causing strains of Staphylococcus ferment mannitol; non-pathogenic strains cannot use mannitol. Is the medium Defined or Complex?