Which protein(s) would be bound to the DNA of the lac operon in the presence of both lactose and glucose? A.) both lac repressor protein AND CRP-cAMP B.)neither the lac repressor protein NOR CRP-cAMP C.)the lac repressor protein D.)CRP-cAMP

Explain how the lac operon is regulated under the following conditions. Include the following terms in your answers: Repressor, Operator, RNA polymerase, CAP site, CAP protein, Promoter A. Lactose and glucose are both present. B. Lactose and glucose are both absent. C. Lactose is present and glucose is absent.

A newly identified protein from the  cells of the Panopyra plant on Pandora was shown to inhibit translation of its target genes by binding to the 5’ UTR of the mRNA and preventing ribosome binding.  A possible way this inhibition may be relieved by an sRNA would be:  Group of answer choices a)The sRNA acts as a silencer, suppressing the inhibitory protein and allowing translation to take place. b)The sRNA acts as a decoy, sequestering the inhibitory protein and allowing translation to take place. c)The sRNA acts as a marker, flagging the inhibitory protein for ubiquitination and allowing translation to take place.

You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan.  Using your knowledge of operons and their regulation   a.Which operons would be functional under these conditions? b.What repressors would be made? (NOTE: name the repressors using their gene names) c.Which repressor(s) would be made in the inactive form? d.Which repressor(s) would be made in the active form? e.Which repressor(s) under these conditions can bind the operator sequence? f.Which repressor(s)under these conditions cannot bind the operator sequence?

Bob determines that Red/pink protein is synthesized at much higher amounts than the Brown protein; Bob thinks that this is how the burger always is perfectly medium-rare (pink/red in the interior of the patty, with a small amount of brown at the burger’s surface). Bob also knows that R and B both are transcribed from the single operon. Briefly describe the biological mechanism allowing for accumulation of more R than B protein.

1) A. What is the difference between a repressible vs, an inducible operon?   B. Using diagrams and words, describe how the Lac Operon is regulated by different levels of lactose and glucose. Be sure to show what’s happening at the molecular level, including the roles of lactose, glucose the repressor protein, cAMP and CAP.

There is a mutation in sequence of the lac operator that prevents the lac repressor from being able to recognize and bind to it. What would the status of transcription of the lac operon be under each of the following circumstances? Explain your answers.   a-Lactose present, glucose present b-Lactose present, glucose absent   c-Lactose absent, glucose present   d-Lactose absent, glucose absent

If β-galactosidase is expressed when lactose is absent, what does this suggest about the lac operon?   a)  There is a mutation in the repressor protein   b)  There is a mutation in the catabolite activator protein   c)  There is a mutation in the promoter region   d)  There is a mutation in the luxI protein

There is Hyaluronic acid synthesis occuring in Group X Strep and it is controlled by an operon with 3 genes, called hasXYZ. Based on the 3-line diagram model,  a. How many ribosome binding sites are there for the protein? b. How many promoters are there for the genes? c. How many start codons are there for the protein? d. How many RNA Polymerase binding locations are there for the genes? e. How many proteins will be fully functional? f. How many mRNA strands are made?