Which of the following is NOT a function of the loading buffer used in SDS-PAGE? A) It contains a dye, letting you track the progression of electrophoresis samples B) It keeps the sample from floating away out of the gel well C) It measures the protein concentration of your samples D) It denatures proteins to turn them into linear chains of amino acids

When running a gel, the lane loaded with a negative control sample should will always show a band that lines up with the experimental band, regardless if the sample is contaminated. not show a band, which indicates the sample is contaminated. show a band, which indicates that the sample is not contaminated. not show a band, which indicates that the sample is not contaminated.

A number of tests are used to identify a bacterial pathogen taken from human patients.Research and describe a biochemical test that is used to help determine of the genus and species of a bacteria. What bacterial types are usually identified with the use of the test you chose?

SDS is required to unfold proteins during SDS-PAGE electrophoresis and it is included in both the loading and running buffer. If SDS is not included in the running buffer.... the proteins in your sample may partially renature thus affecting migration. the gel will not conduct current in the absence of SDS. there is no effect on the samples. the proteins in your sample will be graded.