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- Which of the following indicates buffering in the titration given in the image below? 12 11 10 Point 1 Point 2 8 7 pH Region 1 Region 2 6 4 3 Cocoo 2 + + + + + 10 15 20 25 30 35 40 45 50 ml of 0.1MHCIWhich of the following is true regarding microbial tests that lead to color change in tubes used to characterize bacteria? (more than one answer may be correct) OUrea is degraded by urease, causing a rise in pH Urea is degraded by urease, causing a rise in acid level Production of carboxylic acids causes a rise in pH Production of carboxylic acids causes a drop in pHBased on the following data, which bacteria is most likely a human pathogen? Bacteria E.coli S. marcescens B. Stearothermophilus S. marcescens E. coli B. Stearothermophilus None are human pathogens Absorbance Val 0.01 0.04 0.01 4 C
- As shown in the image below, 10 µl of a stock solution (tube #1) are serially diluted into 0.990 ml of buffer. a. What is the dilution factor for this serial dilution? b. If the concentration of the stock solution in tube #1 is 0.8 M, what is the concentration of the solution in tube #3? 10 ul 10 l 10 0.990 ml SM buffer 0.990 ml SM buffer 0.990 ml SM buffer Tube #1 tube #2 tube #3 tube #4 For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).the scientist is studying the effect of water purification tablets on the creek water in her local area. She collected several different 2 L samples from a creek. For each sample, she measured the number of coliform group bacteria colonies in the water initially, and then added a water purification tablet and measured the number of coliform group bacteria colonies over time. She found that the following equation was a good fit to the data: C(t) = 80e−0.05t + 8(t + 1)−0.5.Her model says that C(t) is the number of coliform group bacteria colonies at time t, where t is measured in minutes since the tablet was added to the water. (a) According to this model, how many coliform group bacteria colonies would initially be in a 2 L sample of the water? background information: The quality of drinking water is determined by tests for coliform group bacteria, which are organisms found in soil and in the intestinal tract of warm-blooded animals. Coliform group bacteria are not considered to…Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
- a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.How many grams of sodium chloride is required to render 80ml of 2% solution of ascorbic acid isotonic with blood. using The sodium chloride equivalent of drug = 0.18 Select one: O a. All of the answer is wrong. Ob 0.256 gm O c. 0.576 gm Od 0.432 gmThe pH in the rumen of corn-fed cows is imbalanced and harbors E. coli bacteria (such as strain O157:H7) which is especially pathogenic to humans. True False
- You come across a gram positive bacterium with the following nucleotides: ATTCGATCGGTATAT. This bacterium most likely is a [ Select one] Firmicute Actinobacterium Proteobacterium You know this because of the [ Select one ] A+T G+C A+C G+TBased on the following image, which concentration of antibiotic would be considered the minimum bactericidal concentration (MBC)? a. 8 ug/mlb. 16 ug/mlc. 25 ug/mlA pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?