Which of the following is incorrect about the Michaelis-Menten plot? a. The y-axis is velocity and the x-axis is [substrate] Ob. It is based on E + S to reversibly form ES and then this to form E + P C. The data is plotted using the Michaelis-Menten equation Od. It has a sigmoidal shape

For the following problem indicate the values for each part of the equation and solve for the unknownvalue:Peter adds 200 μL of bacteria to a tube containing 2 mLs of nutrient broth and then adds 300 μL of acertain disinfectant. The final concentration of the disinfectant in this tube after adding it is 5%.C1=C2=V1=V2=

SUBSTRATE CONCENTRATION [S] µM INITIAL VELOCITY V0    s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and initial velocity (Vi) where [S] has the unit µM, and Vi has the unit mM/s. (ii) The table provided is the enzyme kinetic data for your mutated enzyme, whereby Vi was expressed using the unit ∆A(405 nm)/s. Using the standard curve, express Vi with the unit mM/s rather than ∆A(405 nm)/s. Place your answer in the table above alongside the appropriate [S]. Hint: To answer this question you need to use this standard curve equation=0.0419x (The slope of the line is= 0.0419) (iii) The unmutated form of your protein has a Km of 25 µM and a Vmax of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table above. Based on these data, what is Vmax? Km? and…

You are given a pure protein sample to characterize and provided the following information: Its molar extinction coefficient, ε280, is 0.25 liters micromole-1 cm-1 in both the folded and unfolded form            Its ΔGo for unfolding is 1.5 kcal/mol at 37o  (where RT = 0.59 kcal/mole) A) Using a 0.5 cm pathlength cell, you measure the absorbance at 280 nm of a 20-fold dilution of your pure protein in solution (by this, we mean that 50 ul of the protein sample was diluted to a final volume of 1 ml) and find A280 = 0.40. What is the original concentration of the protein before dilution? B) What is the concentration of the unfolded form of the protein in your sample?