1. Ifa Lineweaver-Burk plot gave a line with an equation of y=0.25 x +0.34, what are the values of KM and Vmax if the substrate concentration is in mM and the velocity in mM/s?
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- During transfer of liquids using a micropipette: What would it mean if the mean is very close to the expected value but your standard deviation is high ?in the noncompetitive inhibtion If Km = 2 mM, and Vo = 100 umol.s when [S] = 2 mM, what is the velocity Vo for the reaction when [S] = 18 mM ? 360 umol.s 180 umol.s 90 umol.s 200 umol.sFrom the equation of a lineweaver-burke plot, calculate Km and Vmax. Show your work and include units. The equation is y=393.4x +8.4337
- The equation of the double reciprocal plot is y = 0.5294 x + 1.4960. What is the value of vmax (in M/s)? The substrate concentration is given in units of molarity (M) and reaction velocity has units of molarity per second (M/s). (Report to three significant figures)For Table 1: calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.Ismail analyzed the triester content in an esterified oil using the gas chromatographic technique. The measurements for triester contents are 33.27, 33.37, and 33.34%. Calculate: (i) the average triester content (ii) the standard deviation, s for triester content (iii) the relative standard deviation for the triester content
- 1.In a kinetics data set, in case a substrate concentration is given [S] in uM and inhibitor concentration [I] uM, initial velocity (Vo) in umol/min , Km , Vmax, apparent Km, apparent Vmax, and Ki, is there any way of finding Kcat ? 2.Which formula would be used in that case? What would the process be for finding Kcat?Consider the titration of 50.00 mL of 0.160 M NH3 with 0.200 M HCl. Calculate the pH at the following volumes of the titrant: VT = 0.00 (Initial Stage) VT = 20.00 VT = 30.00 VT = 40.00 (Equivalence Point Stage) VT = 45.00 Kb = 1.76 x 10-5How does one measure Km using a Lineweaver-Burk plot? 1. By determining the reciprocal of the y-intercept 2. By determining the negative reciprocal of the x-intercept 3. By determining the slope and multiplying it by kcat 4. By determining the saturation point
- Thank you for helping me. I have to questions. In solution B, if we choose two other points on the graph for calculating the slope, wouldnt the values for Km/Vmax be different than 6.1? Why did you choose exactly those points on the graph? Another thing, I understand the method you used to get to Km/Vmax= slope, but how did you then get the value for Vmax to be 10 mM/s??1. What volume of diltiazem HCl will you add? 2. Do you need to remove any of the 0.9% NS to account for the added volume of the diltiazem? 3. Assuming that the rate does not change, how long will this bag last for?If Km = 2 mM, and Vo = 100 umol.s when [S] = 2 mM, what is the velocity Vo for the reaction when [S] = 18 mM? 360 umol.s 180 umol.s 90 umol.s 200 umol.s