Which of the following experiments can be used to determine the localization of a protein in living cells? a. Isoelectric Focusing gel electrophoresis b. Mass spectroscopy of a MALDI-ionized protein c. X-ray Crystallography of a protein d. NMR study of a 15N-labeled protein e. Confocal microscopy study of a GFP-tagged protein
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
Which of the following experiments can be used to determine the localization of a protein in living cells?
Isoelectric Focusing gel electrophoresis
Mass spectroscopy of a MALDI-ionized protein
X-ray Crystallography of a protein
NMR study of a 15N-labeled protein
Confocal microscopy study of a GFP-tagged protein
Confocal microscopy is an optical imaging technique used for increasing optical resolution and contrast by using a spatial pinhole to block out-of-focus light during image formation.
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