During the lysogenic cycle of lambda (A) phage the phage genome is integrated in the host chromosome where it is passed on to daughter cells through binary fission. the phage DNA is copied and accumulates in the cytoplasm. Then a trigger causes capsid proteins to be made and phages are assembled. the product of one prophage gene is made and it activates most of the other prophage genes. the viral genes immediately turn the host cell into a lambda-producing factory. Then the host lyses.
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- Which of the following statements describes the lysogenic cycle of lambda (A) phage? After infection, the viral genes immediately turn the host cell into a lambda-producing factory, and the host cell then lyses. Most of the prophage genes are activated by the product of a particular prophage gene. The phage DNA is incorporated into the bacteria DNA The phage DNA is copied and exits the cell as a phage. The phage DNA get destructed by the host bacteria DNAphage can be "induced" as a result of DNA damage. Consequences of induction include. Temperate phage, e.g lambda phage; lytic cycle and transduction Lytic T4 phage, assembly, conjugation O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation Temperate phage, e.g. lambada phage, prophage formation, transformation O T4 phage, lytic cycle, specialized trasnductionWhich of the following is true of Generalized transduction? O creates defective virions that are missing the entire phage genome phage and host DNA are both packed in the capsid by error Cha type of Hirgone transfer Frequency is I in 10¹ O occurs during the lysogenic cycle
- Bacteriophage T4 lacks its own RNA polymerase.How do T4 genes get expressed or converted to mRNA?What host barriers must be broken before release ofvirions from the host cell?What is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisHost DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?
- Experimentally, when an E. coli bacterium already has a λ prophageintegrated into its chromosome, another λ phage cannot usually infectthe cell and establish the lysogenic or lytic cycle. Based on yourunderstanding of the genetic regulation of the phage λ reproductivecycles, why do you think the other phage would be unsuccessful?When a temperate phage infects a bacteria cell it O immediately undergoes the temperate lifecycle. O immediately integrates its genome into the host cell genome; when conditions are harsh, the phage excises out of the host chromosome and undergoes the lytic lifecycle. O immediately undergoes the lytic lifecycle. O can either immediately undergo the lytic lifecycle or immediately undergo the lysogenic lifecycle, and it usually undergoes the lytic lifecycle.Indicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1. The host cell is lysed. (Click to select) v 2. The genetic material of the phage is copied. (Click to select) v 3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v 4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v 5. The integrase gene is turned on.: (Click to select) v (Click to select) lytic lysogenic both
- Compare and contrast the mechanism of replication of T4 vs. filamentous phages.Compare and contrast the lysogenic and lytic cycles of phage λ.This gene is responsible for making the protein that catalyzes the recombination of the phage genome into the host bacterial chromosome is: excise integrase attP attB