When plasmids are used to produce a desired protein O the plasmids are inserted into the bacterial chromosome. O the plasmids multiply and produce the protein outside of the bacterium. O the desired gene is inserted into the plasmid, and the plasmid is returned to the bacterium by transformation. O the bacterial genome and plasmid are inserted into the genome of the cell containing the desired gene (often the cell of a plant or animal).

When plasmids are used to produce a desired protein O the plasmids are inserted into the bacterial chromosome. O the plasmids multiply and produce the protein outside of the bacterium. O the desired gene is inserted into the plasmid, and the plasmid is returned to the bacterium by transformation. O the bacterial genome and plasmid are inserted into the genome of the cell containing the desired gene (often the cell of a plant or animal).

Biology 2e

2nd Edition

ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter17: Biotechnology And Genomics

Section: Chapter Questions

Problem 1VCQ: Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner...

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Pair the appropriate item with its purpose in the production of insulin a DNA containing the gene for insulin. b Restriction enzymes and DNA ligase c PBR322 (a plasmid) d Lac promoter (regulatory sequence) e IPTG Triggers gene expression Provides sequences needed for RNA polymerase to bind and transcribe. Provides a means of getting the DNA into the bacterium and keeping it there. Allows DNA to be cut and put back together in order to produce the needed construct. Contains the sequence the bacterium needs in order to produce insulin.
Figure 17.7 You are working in a molecular biology lab and, unbeknownst to you, your lab partner left the foreign genomic DNA that you are planning to clone on the lab bench overnight instead of storing it in the freezer. As a result, it was degraded by nucleases, but still used in the experiment. The plasmid, on the other hand, is fine. What results would you expect from your molecular cloning experiment? There will be no colonies on the bacterial plate. There will be blue colonies only. There will be blue and white colonies. The will be white colonies only.
Which of the following single-stranded DNA molecules may possibly be a restriction enzyme target cut site in the double-stranded state? O ATGCCGTA GTATCTAT OATGCTACC OGTCATGAC
In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. indicate which plasmids were successfully digested by the endonuclease. act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.
You clone a fragment of DNA into the middle of the lacZ sequence of a plasmid, introduce the plasmid into bacteria, and then plate the bacterial colonies with ampicillin and X-gal. If you select blue colonies rather than white colonies, you will isolate bacterial colonies that O contain only the cloned piece of DNA. O contain the plasmid plus a cloned piece of DNA. O are not ampicillin-resistant. O contain only the plasmid. X
You cut a plasmid with a restriction enzyme that cuts it into three pieces-one fragment is 280 base pairs, one fragment is 800 base pairs, and one fragment is 1400 base pairs. You carry out electrophoresis of the digested sample. Which of the fragments will be closer to the top of the gel where the sample was inserted? O The 280 base pair fragment. O The 1400 base pair fragment. O The 800 base pair fragment. O They will all be near the top because they all have sticky ends.
One of the frist steps in isolating plasmid DNA via mini-prep is to pellet the cells after O/N culture and then resuspend them in buffer P1. What's the point of pelleting the cells just to resuspend them again? To concentrate the cells P1 is a buffer, preventing small changes in pH O This step is not absolutely necessary P1 begins the lysis process D Question 2 What would happen if we didn't centrifuge the tube containing lysed cells after adding neutralization buffer and instead just added right to the column? O We could fihish the prep but we would have protein/RNA contaminants at the end O The precipitate is less dense than water so it shouldn't get in the way since it floats on top O The precipitate would obstruct the column and our prep is ruined O Our yield would be reduced, but we'd get something
The PET28 plasmid is cut with the Xhol and Ncol restriction enzymes. A 200 bp gene, also cut with Xhol and Ncol, is ligated to the large segment of the plasmid. In the new plasmid, what is the location of the Xbal restriction site? O 335 bp O 535 bp 397 bp O 197 bp
You transform bacteria with a plasmid carrying the ampicillin-resistance gene ampR. How would you determine which bacteria took up the plasmid? O Bacteria containing the plasmid would be able to grow in the absence of ampicillin. O Bacteria containing the plasmid would be able to grow in the presence of ampicillin. O Bacteria containing the plasmid would be able to produce ampicillin. O Ampicillin-resistance is necessary for the plasmid DNA get into the bacteria.
E. coli cells usually have only one copy of the F plasmidper cell. You have isolated a cell in which a mutationincreases the copy number of F to three to four per cell.How could you distinguish between the possibility thatthe copy number change was due to a mutation in theF plasmid versus a mutation in a chromosomal gene?
In a hypothetical situation, the gene for tetracycline resistance is located on a plasmid within a particular bacterium. This bacterium readily forms a pilus and donates one strand of the plasmid to another bacterium. What do you know about the bacterium with the tetracycline resistance? O It cannot receive a plasmid from another bacterium. O It must have the F factor gene because it can perform conjugation. O If it donates the plasmid, it will lose its own tetracycline resistance. O It has the Hfr gene and it can participate in transformation.
You have set up a recombinant DNA experiment using the plasmid PBR322 as the vector (see plasmid below). You use the BamHI restriction site on the plasmid to insert the target DNA. The plasmid is then used to transform E.coli colls Is the following statement True or False? Growth of the transformed cells on agar containing both ampicillin and tetracycline will eliminate any cells that do not contain a plasmid. Clal Hindlll EcoRI Pvul BamHI Pstl amp tet PBR322 -Sall ori rop Pvull True False