In bacterial transformation, the purpose of having antibiotic within an agar plate is to: Select one: O confirm which plasmids been have successfully ligated with a gene of interest. isolate bacteria which have been successfully transformed with the plasmid. O indicate which plasmids were successfully digested by the endonuclease. O act as a substrate which will be cleaved and produce a blue product when ligation is unsuccessful. show which plasmids contain the lacZ gene.

Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.

Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.