To identify mutant genes, a wild-type bacterial genomiclibrary in plasmids is used to transform the mutant bacterialstrain. A transformed ________ in which the mutantphenotype is rescued to wild type likely harbors a plasmidcontaining a wild-type copy of the corresponding gene

Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The only thing sheknows is that afg was cloned into the vector using a single restriction enzyme, BstBI. Margaret orders theBstBI enzyme from NEB, so she can cut the plasmid and confirm the presence of the insert. On theNanodrop, she measures the concentration of her plasmid to be 401 ng/μL. Describe how she would setup and perform this reaction if she wanted to digest 2.0 μg of the plasmid.

Linear chromosomes would shorten after each DNA replication because the gaps created by the removal of primers are not filled. Telomerase is an enzyme that fills this gap using an RNA template (a conceptual diagram of the function is shown below; actually this is a protein-RNA complex).   Most human cells do not have the telomerase activity and thus can divide only a limited number of times.  _________ exceptionally have telomerase activity because these cells are supposed to pass the complete DNA to the next generation. By Sierra Sciences, LLC - Sierra Sciences, LLC (uploaded from intranet), CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=7988056      Brain cells     Germline cells     Cancer cells     Hepatic cells

If a mutation that inactivated telomerase occurred in acell (telomerase activity in the cell = zero), what do youexpect the outcome to be?

true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme cuts between the second A and the C, this will produce a "sticky end," which is useful for cloning into a plasmid vector.

Please don't provide handwriting solution

This answer is copied, a sign of plagarism, I need a explanation not from the internet

The mutagen that most often causes thymine dimers is/are ________. Group of answer choices ultraviolet light reactive oxidants (free radicals) 5-bromouracil ethylmethane sulfonate acetaldehyde

______________ is a kind of spontaneous mutation that results from a short-term, reversible phenomenon that leads to abnormal base pairing during DNA replications, followed by another round of DNA replication which results in one of the two daughter DNA molecules containing a point mutation. Group of answer choices Tautomeric shift Frameshift mutation Nonsense mutation Pyrimidine dimer Conditional mutation

I’m having trouble finding which answer would be acceptable can you please help?