Q: What is the purpose of transgenesis
A: Since you have asked multiple question, we will solve the first question for you. If you want any…
Q: What is the advantage to transferring proteins bands from a gel to nitrocellulose during the western…
A: The western blot is a broadly utilized scientific procedure in molecular biology and immunogenetics…
Q: In a gel electrophoresis, what do the lanes mean? Please desribe.
A: Gel electrophoresis is a procedure that is used for the separation of the DNA fragments based on…
Q: Explain how bacterial conjugation has been extensively used in determining the complementing gene…
A: Bacterial conjugation is one of the most common ways for genetic information to spread between…
Q: Why is pure culture and culture collection important in identifying novel species of bacteria?
A: A pure culture is a population of cells or multicellular organisms growing in the absence of other…
Q: Explain the steps of preparing a cell culture stock from a T25 flask.
A: Cell culture is the specialized process used in molecular biology to remove cells from plants and/or…
Q: The cath sample was both filtered to remove all bacteria, and then treated with DNase. Based on…
A: A cath or catheter is a thin hollow tube, which is a medical equipment that is used for various…
Q: What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a…
A: Recombinant DNA technology (rDNA technology) is very helpful for analysing the entire genome of a…
Q: Contrast the use of plasmid vectors with that of BAC orYAC
A: Cloning vectors are also commonly called as vectors. They play an important part in molecular…
Q: you transform bacteria with the pGLO plasmid. What do you need to add to the media to get them to…
A: Green Fluorescent Protein (GFP) is obtained from jelly fish (Aequorea victoria). It has excitation…
Q: What is the purpose of using DNA polymerase from Thermus aquaticus for the polymerase chain reaction…
A: Thermus aquaticus is a heat tolerant bacterium .DNA polymerase used from Thermus aquaticus is heat…
Q: Trace the route of the nutrients PO43- carried by river runoff to a seagrass ecosystem. Indicate the…
A:
Q: Agraobacterium mediated transformation is a useful gene transfer technique but still we can’t use…
A: Agrobacterium species harboring tumor-inducing (Ti) or hairy root-inducing (Ri) plasmids cause crown…
Q: You have two strains of E. coli, Strain I and Strain II. These strains were also co- cultured on a…
A: Antibiotics are those substances that kill or prevent the growth of bacteria. Antibiotics can be of…
Q: Please create a dichotomous key using these tests to isolate each bacteria strain.
A: The microorganisms distinguished by many methods and gram staining is one of the most important…
Q: Why is the catalase test useful for the differentiation of staphylococci from streptococci?
A: Bacteria are classified into two categories gram-positive bacteria and gram-negative bacteria.…
Q: What is one way to determine whether a bacterial culture has receiveda recombinant plasmid?
A: Plasmids are small, extra chromosomal, and double stranded DNA structures that can replicate…
Q: Trace the route of the nutrients PO43- carried by river runoff to a seagrass ecosystem. Indicate the…
A: Phosphate forms the most important ingredient in the living organisms. The DNA which consists of the…
Q: Why is the use of auxotrophy not a good method for controllingthe growth of a genetically modified…
A: Genetically modified organisms are those living organisms which have been genetically modified by…
Q: State the differences between the plasmid in Figure 1 with pBR322 in terms of their sercening…
A: According to the question, we have to state the differences between the plasmid in figure 1 with…
Q: How does diagnostic method for detecting Covid-19 using RT-PCR work?
A: RT–PCR also called Real-time PCR (polymerase chain reaction) is a nuclear-derived method or test…
Q: Why are transgenicsconsidered a threat to theenvironmental safety?
A: Transgenic organisms or genetically modified organisms are those that have a foreign gene…
Q: A patient comes in with an infection and lab analysis show that the infectious agent is…
A: The bacteria Staphylococcus aureus is a very common cause of minor or major skin infections. This…
Q: What is the addition and limitation of the RT-PCR approach? Keep CoViD-19 in mind
A: Viruses are a nucleoprotein entity which is able to utilize the synthetic machinery of a living cell…
Q: Explain briefly about The Case of Genetically Modified Tomato Paste
A: A plants whose the DNA has been modified using genetic engineering method are known as genetically…
Q: All of the following are true about Restriction endonucleases except fashion
A: DNA fragments are cleaved at particular recognition sites by some enzymes known as Restriction…
Q: How agrobacterium tumefaciens help in transgenic plants production?
A: Transgenic plants are plants in which one or multiple genes from a different species can be injected…
Q: How does penicillin enrichment make it easier to isolate mutants?
A: Bacteria are a form of a prokaryote that can be found on the surface of the Earth. Some are harmful…
Q: isolation and purification of bacteriophages from sewage through enrichment centrifugation and the…
A: Isolation of Bacteriophages Centrifuge the Sewage samples at 12000 x g for 30 minutes. It will…
Q: Given that antibiotic-resistant strains of bacteria are a concern to the general health of a…
A: The antibiotics are a special substances that are released by some bacteria to kill or inhibit the…
Q: define F factor and HFR plasmid, conjugation tube, exconjugant, prophage
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Trace the route of the nutrients PO43- carried by river runoff to a seagrass ecosystem. Indicate the…
A: The biological oxidation of ammonia to nitrite, followed by the oxidation of nitrite to nitrate by…
Q: 4th calculation: total amount (milligram) of DNA in 100g wheat germ assuming an extraction…
A: DNA extraction is a method of separating DNA from cell membranes, proteins, and other cellular…
Q: Based on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which…
A: Antibiotics are substances that have the ability to kill bacteria or restrict their growth. They are…
Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?
A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: what are two differences between agarose gel electrophoresis and SDS PAGE?
A: Agarose gel electrophoresis and the SDS page technique are used to separate the molecules. The…
Q: escribe how the bacteria should have the ability to be recovered back from the infected host for the…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: how do you prepare 200 ml of 1.5 agarose gel in PCR?
A: Agarose is an uncharged, inert substance that does not bind to or react with molecules. Agarose is…
Q: Why is the DNA polymerase used in PCR derived from an extreme thermophile bacteria species rather…
A: Answer
Q: What and where is the restriction site of the lacl gene of K. pneumoniae? What restriction enzyme…
A: The lacZ and lacI genes are transcribed from opposite strands in the lac operon of K. pneumoniae.…
Q: Discuss bacterial conjugation and the three mating types of bacteria (F-, F+, and Hfr).
A: Transfer of genes in prokaryotes occurs through horizontal gene transfer mechanisms such as…
Q: w to achieve carbon dioxide requirement for capnophilic organisms? What is the used of “Thermus…
A: Capnophiles are microbes that can survive in the presence of higher carbon dioxide concentration.…
Q: How Web site, Webcutter are used for generating restriction maps ?
A: Constructing some long-range restriction maps are dependent on making the use of restriction…
Q: Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.
A: F factor can exist as an independent plasmid or integrate into the bacterial cell's genome. It can…
Q: How is bacterial transformation used in biotechnology and How can we tell if we have successful…
A: Bacterial transformation may be referred to as a stable genetic change brought about by the uptake…
when isolating bacterophages, what will be happening if you forgot to do the enrichment phase/stage?
Step by step
Solved in 2 steps
- What is the role of Agrobacterium tumefaciens in plant transformation?Why is Agrobacteriummediated genetic engineering transformation inplants considered as natural genetic engineering?Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plate
- Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.Explain briefly about The Case of Genetically Modified Tomato PasteBased on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which antibiotic? Write the letter of the antibiotic below. G. B. E.
- You are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium? In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium?