What is one way to determine whether a bacterial culture has receiveda recombinant plasmid?

What is the Ti plasmid and how has it been of use ingenetic engineering?

The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel  d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genes

How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?

You take an aliquot of competent cells and transform them with pUC plasmid. What should be the part of the selection medium? a) X Gal b) Ampicillin c) IPTGd) All should be part of the selection medium

Why are plasmids dangerous?

What is pGLO plasmid? How has it been engineered so that the cells that carry it can be detected? Attach a map of it.?

What is a plasmid?

describe the two main parts of a plasmid DNA extraction and purification procedure including the purpose of each step

What is a recombinant microorganism? Discuss examples.