Describe the steps of the chromatin immunoprecipitation assay. What are some of the scientific facts that can be learned through a chromatin immunoprecipitation assay?
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Describe the steps of the chromatin immunoprecipitation assay.
What are some of the scientific facts that can be learned through a chromatin immunoprecipitation assay?
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- What is meant by the term genetic testing? How do testing at theprotein level and testing at the DNA level differ? Describe fivedifferent techniques used in genetic testing.What is recombinant tissue plasminogen activator (rec-tPA)?The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)
- How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed explanationAs shown , several medical agents are now commercially produced by genetically engineered microorganisms. Discuss the advantages and disadvantages of making these agents this way.In recombinant DNA technology, vectors are used to transfer a gene of interest in the host cells. Mention any three features of vectors that are most suitable for this purpose.
- A graduate student isolates two new retroviruses: Virus A and Virus B. To determine whether these viruses could cause cancer, the student infected human cells with each of the viruses and then transfered the infected cells to nude mice to see if the viruses would cause formation of tumors. The student was able to determine by PCR that both viruses are integrated into the genome of the host cells before transplanting cell in mice. The data for this assay are shown in the figure below. Explain how the mechanism of transduction may be different for these two viruses to yield the observed data (limit 4-5 sentences). Retrovirus Transformation Assay % Cells Cause Tumors 120 100 80 60 40 20 0 0 10 -Virus A -Virus B 30 20 Days Post Infection 40It is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?In selecting target cells to receive a transferred gene in gene therapy, what factors do you think would have to be taken into account?