What yield of ATP can be expected from complete oxidation of each of the following substrates by the reactionsof glycolysis, the citric acid cycle, and oxidative phosphorylation?(a) Fructose-1,6-bisphosphate(b) Glucose(c) Phosphoenolpyruvate(d) Glyceraldehyde-3-phosphate(e) NADH(f) Pyruvate

From the complete oxidation of glucose (glucose → 6CO2), how many total NADH electron carriers are produced?

All dehydrogenases of glycolysis and the TCA cycle use NAD* (E° for NAD*/NADH is -0.32V) as electron acceptor except succinate dehydrogenase (which uses FAD (E° for FAD/FADH2 is 0.05V). Based on AG° = -NFEº, show and state (1-2 sentences) why is FAD a more appropriate electron acceptor than NAD* in the dehydrogenation of succinate (consider the E° values of %3D Uptake in Na+ Vmax Uptake in absence of Na+ Vmax substrate K: (mM) Kt (mM) L-leucine 420 0.24 23 0.2 D-Leucine 310 4.7 5 4.7 L-valine 225 0.31 19 0.31 fumarate/succinate (E° = 0.031), NAD*/NADH, and the succinate dehydrogenase FAD/FADH2).

The glycolytic enzymes hexokinase and glucokinase (in human liver cells) catalyze the conversion of: 1,3-bisphosphoglycerate into 3-phosphoglycerate (producing ATP) glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate (producing NADH) phosphoenolpyruvate into pyruvate (producing ATP) fructose-6-phosphate into fructose-1,6-bisphosphate (consuming ATP) glucose into glucose-6-phosphate (consuming ATP)   Which of the following glycolytic enzymes must consume ATP during phosphorylation of its preferred substrate molecule? phosphoglycerate mutase succinate dehydrogenase phosphoglycerate kinase phosphofructokinase pyruvate kinase

Briefly describe the biological rationale for each of the following allosteric phenomena: (a) activation of pyruvate carboxylase by acetyl-CoA; (b) activation of pyruvate dehydrogenase kinase by NADH; (c) inhibition of isocitrate dehydrogenase by NADH; (d) activation of isocitrate dehydrogenase by ADP; (e) inhibition of α-ketoglutarate dehydrogenase by succinyl-CoA; (f) activation of pyruvate dehydrogenase phosphatase by Ca2 +.

The first step of gluconeogenesis involves the carboxylation of pyruvate and has a large negative delta G (i.e very exergonic). Explain how it is possible that this step can be exergonic if we know that adding CO2 onto molecules is fundamentally a destabilizing phenomenon?

Arsenate can replace inorganic phosphate in the reaction catalyzed by glyceraldehyde-3-phosphate dehydrogenase, causing glyceraldehyde-3-phosphate to be directly converted to 3-phosphoglycerate(NADH is still formed). Which of the following is absolutely true in case of arsenate poisoning -Glycolysis will stop - Glycolysis will still proceed with no net ATP gain - Glycolysis will not generate any form of energy  - Glycolysis will still proceed, but with a net consumption of ATP - Glycolysis will proceed with lactate as end product

How many molecules of NADH are produced when one glyceraldehyde- 3-phosphate is metabolized through glycolysis and Krebs cycle?