How would I be able to calculate alpha, beta, and gamma diversity from sites A, B and C?

a) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.   b) Much to the dismay of the researcher with whom Sam is working, Sam is not only forgetful, but he is also lazy! Rather than devoting the necessary hours to conducting his assigned interrupted mating experiments, Sam has decided that his time would be better spent modifying the F plasmid so that the experiments wouldn’t require quite so much time. The diagram below shows a map of the E. coli F plasmid, similar to that shown in lecture. Sam is determined to “get rid of some of the excess DNA” present in the plasmid. Please keep in mind that the PI in the lab in which Sam works utilizes these F plasmids for interrupted mating experiments between Hfr and F- cells as well as for interrupted mating experiments involving F+…

What are the function of southern blotting in DNA FINGERPRINTING?

What did Rosalind Franklin do when she saw Watson and Crick’s first model? Why?

How would you ascertain if your restriction experiment was successful?

For the hybridization step in the Southern blotting, the hybridization was done at 85 degrees Celsius instead of 58 degree Celsius. Explain how this would affect my results.