What are the approximate sizes of each band and their relative brightness after clean up pcr?

Q: What is the functional difference between regular PCR and qPCCR?

A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…

Q: How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA…

A: DNA microarray is a chip-based technique where a collection of microscopic DNA spots attached to a…

Q: What would be the final primer concentration if 0.5 ul of 10 uM primers were ad a PCR reaction with…

A: Introduction: A primer is a short strand of DNA or RNA that is usually about 18-22 bases and serves…

Q: why different thermal conditions are necessary for successful PCR?

A: DNA polymerase is an enzyme that naturally polymerizes nucleotides to polynucleotide strands. The…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: How VNTRs are useful for DNA profiling ?

A: DNA profiling is defined as technique which involves specific DNA patterns known as profiles…

Q: primer

A: PCR- it stands for the polymerase chain reaction. it is used to make many copies of a specific…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: How does magnetic bead DNA extraction work?

A: DNA extraction is the method of isolating DNA by disrupting the cell wall and nuclear membrane with…

Q: What is real-time PCR?

A: Besides tools, there a several techniques which are used in recombinant DNA technology. These…

Q: What are the function of autoradiography in DNA fingerprinting?

A: Deoxyribonucleic acid (DNA) fingerprinting is laboratory technique that is used to establish a link…

Q: how to find approximate sizes of each band and their relative brightness in pcr?

A: Polymerase Chain Reaction ( PCR )--  It is a common laboratory technique to form many copies (…

Q: Describe the Amplified Fragment Length Polymorphisr (AFLP) method. Give the advantages and…

A: AFLP is a technique used to detect polymorphisms in DNA when no information about the genome is…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: How is fluorescence in situ hybridization (FISH) used to producea spectral karyotype?

A: Fluorescence in situ hybridization (FISH) is a technique that is used to detect and locate a DNA…

Q: What are some of the observations and conclusions that can be made from gel bands for 16s PCR

A: Answer :- Here are some observations :- 1) Increase hardening temperature by increments of two…

Q: What would a yellow green spot on the microarray mean?

A: The microarray is a technique to determine the expression of thousands of genes at the same time.…

Q: Estimate the concentration of your PCR product (amplicon) in ng/μL by visual comparison. 10 μL of…

A: Polymerase chain reaction (PCR) is a technology that is used to target specific DNA fragments and…

Q: How does Sanger sequencing differ from PCR?

A: Sanger sequencing and PCR are two unique molecular biology techniques, however they perform…

Q: suppose ampliTaq gold is being used to eliminate undesired amplicons in PCR. if the pH of the…

A: During PCR process amplicon is a stretch of DNA or RNA which is the source of amplification or DNA…

Q: What are the criteria for “good” primers in a PCR reaction?

A: Polymerase chain reaction (PCR) is the method that is widely used to make billions of copies of a…

Q: Describe why different thermal conditions are necessary for successful PCR?

A: DNA polymerase is a natural polymerization enzyme that turns nucleotides into polynucleotide…

Q: If DNA is extracted from the juvenile leaves of mint plant. Then how to check the quantity and…

A: Gel electrophoresis is a technique used widely to separate the DNA molecule based on its charge and…

Q: Prepare an X µL PCR reaction mixture and set thermal conditions for gene amplification?

A: PCR is a strong amplification technique that can produce a large amount of a particular DNA segment…

Q: What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis…

A: Sanger sequencing method is chain terminating method.

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: What is mass fragmentography? How is this performed?

A: Mass fragmentography is also known by the name mass fragmentometric analysis or mass fragmentography…

Q: What is the formula used to calculate the number of DNA molecules which will be created for a given…

A: PCR or polymerase chain reaction is a technique of DNA amplification. Here a small amount of DNA is…

Q: What is meant by the term DNA fingerprinting?

A: Every cell of a human possesses its genetic material i.e. DNA enclosed inside a membrane-bound…

Q: What is Spectral Karyotyping.?

A: In spectral karyotype the homologous pairs of chromosomes are manipulated in way that they have…

Q: What is DNA fingerprinting? Define and differentiate VNTR and Probe and what is the applications of…

A: Step 1 DNA or deoxyribonucleic acid occurs in living systems and can function as genetic materials.…

Q: How does adjusting Mg2+ concentration affect the PCR yield and specificity? How does adjusting…

A: Introduction: Polymerase chain reaction involves the ampliifcation of a particular piece of DNA to…

Q: If the PCR negative was positive what would that mean?   If the PCR positive was negative what…

A: PCR is also known as polymerase chain reaction is used to amplify the DNA and get accurate results…

Q: Gel Electrophoresis Separates DNAFragments According to which thing?

A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…