Which is a limitation of using massively parallel sequencing ?

Q: What would be some major impediments to genetically modifyinghuman embryos with CRISPR?

A: Genome editing tools are those tools which help in making changes into the genome of an individual.…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: diiferentiate  between PCR and Sanger sequencing?

A: PCR (polymerase chain reaction is a technique through which a DNA segment is copied to million folds…

Q: What is Sanger sequencing used for?

A: DNA sequencing is defined as a process in which DNA’s nucleotide sequence is determined. The main…

Q: How was the first Copy Cat was cloned from a kitten?

A: Cloning is the practice of creating organisms, either spontaneously or intentionally, with similar…

Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…

A: Introduction: A laboratory method used to recognize the expression of thousands of genes…

Q: Who proposed shotgun sequencing?

A: The method used for the sequencing of random DNA strands is genetics, shotgun sequencing.  This is…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What is synthetic or artificial genome ?

A: A synthetic genome is an artificially built genome that is formed by processes involving genetic…

Q: What is a cloning vector? Give two examples.

A: A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into…

Q: What is exome sequencing ? Why it is important ?

A: The exome has historically been described as the sequence in the genome that includes all exons of…

Q: Why are the proteins cleaved into small fragments for protein sequencing?

A: Proteins are the end product of the molecular translation that codes the information present in the…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: Why is the technique used to clone mammals called somatic cellnuclear transfer?

A: A somatic cell is a cell that takes part in the composition of the body of an organism and divides…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: What are the two methods used for sequencing in Human genome project.

A: Answer- Human genome project was the mega project that aimed for sequencing of whole human genome.

Q: What are the steps in shotgun sequencing?

A: Shotgun sequencing is a laboratory method which is used to determine the sequence of DNA of an…

Q: What are the advantages of Next Generation Sequencing?

A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…

Q: Explain what is  Flow cell DNA sequencing ?

A: What is DNA sequencing? It is the process of finding out the order of nucleotides i.e. adenine,…

Q: What does massively parallel sequencing mean?

A:

Q: Give a step by step method and diagram of how reversible terminator sequencing works.

A: The reversible terminator sequencing or Illumina dye sequencing is a technique used to determine the…

Q: How to find unknown gene and design primers present using whole genome sequence?

A: Step 1 Whole genome sequencing is the process that regulates the complete deoxyribonucleic acid…

Q: What is a read (Sanger sequencing)?

A: DNA sequencing is the process of determining the sequence of nucleotides (adenine, guanine,…

Q: Contrast and compare the advantages and disadvantages of the Sanger method with next-generation…

A: Sanger and NGS are two sequencing methods for analyzing the sequences of DNA. The benefits of Sanger…

Q: Why is GenBank important and What is GenBank format?

A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…

Q: What does whole genome sequencing reveal?

A:

Q: What is shotgun cloning or shotgun sequencing ?

A: Cloning is the process of creating an identical copy of something. In biology, it refers to the…

Q: What class of genes is used in MLST analyses?

A: MLST refers to multilocus sequence typing. MLST involves higher frequency of recombination in…

Q: How can libraries be used to locate a specific gene of interest?

A: Scientists use genetic engineering methods to create two different types of DNA libraries. These are…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: Chromosomal microarray, CGH, and Exome sequencing: compare and contrast!

A: In molecular biology many techniques are used for different purpose like PCR is used to generate…

Q: What will be the result if we resequence the same samples contributed to the reference genome and…

A: Many hereditary disorders may now be tested for through "genetic testing". Some diseases,…

Q: How does the shotgun approach differ from the whole genome sequencing?

A: Biotechnology is concerned with exploitation of biological agents or their components for generating…

Q: What is the difference between a map-based approach to sequencing a whole genome and a whole-genome…

A: Deoxyribonucleic acid (DNA) is a double stranded helical genetic material containing thousands of…

Q: What are steps of sequencing?

A: Introduction: Considering that it is DNA sequencing.  DNA is the genetic material of a living cell.…

Q: What is next generation sequencing technology?

A: DNA sequencing is used to determine the exact arrangement of the nucleotide bases adenine (A),…

Q: What is a Sanger Sequencing ?

A: The term DNA sequencing encompasses biochemical methods for determining the order of the nucleotide…

Q: Provide five advantages of Next Generation Sequencing? and explain each of these advantages.

A: Here I will discuss about the advantages of next generation sequencing.

Q: Explain how Sanger sequencing works

A: Genome sequencing: genome means total gene content present in the organism and sequence means to…

Q: What is a microarray? How can it be used to obtain information about gene function?

A: A gene is a specific nucleotide sequence in RNA or DNA. It is generally located on a chromosome.…

Q: "Whole-Genome Sequencing Is Widely Used for Sequencing and Assembling Entire Genomes". Explain this…

A: Whole genome sequencing It reveals an organism's complete DNA make-up ( allowing us to better…

Q: Explain the use of Annotation to Identify Gene Sequences ?

A: The attachment of biological information to the gene sequence is known as genome annotations. Three…

Q: What can exome sequencing do for you?

A: The sequencing of the nucleic acid is the process through which the exact sequence of the nucleotide…

Q: What is single molecule sequencing in real time (SMRT) ?

A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.