What polypeptides would be formed from the sequence UCAATGGGGUUUAUAGCG… (there are bases after the last G but we don’t know what they are so there may be some ambiguity for the last codon with regards to the amino acid possibilities)   if the translation frame had UCA as the first codon:   if the translation frame had CAA as the first codon:   if the translation frame had AAT as the first codon:

Given the following protein, which of the following sequences of TEMPLATE strand DNA would code for it? Pay attention to the polarity of the polypeptide and the strands of DNA that you choose. Use the codon chart to the right. AUG = met AAA = lys GCU = ala | CUU = leu ACU = thr -lys - thr - ala - leu - met (amino end) 5' TAC GAA CGA TGA TTT TAC ATT 3' 5' ATG CTT GCT ACT AAA ATG TAA 3' (carboxyl end) met 5' TAC TTT TGA CGA GAA TAC ATT 3¹' 3' TAC TTT TGA CGA GAA TAC ATT 5¹ 5' ATG AAA ACT GCT CTT ATG TAA 3¹ 3 TAC GAA CGA TGA TTT TAC ATT 5'

You want to translate a polycistronic bacterial MRNA in eukaryotic cells. You remove all stop codons except the last stop codon in the bacterial DNA before inserting it into the genome of a eukaryotic cell. Can you get a functional protein using this method? Yes or No. Briefly justify your answer.

Which of the molecules of RNA is the most likely to fold into a specific structure as a result of intramolecular (within itself) base-pairing?  Explain. 5′-CCCUAAAAAAAAAAAAAAAAUUUUUUUUUUUUUUUUAGGG-3′ 5′-UGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUGUG-3′ 5′-AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA-3′ 5′-GGAAAAGGAGAUGGGCAAGGGGAAAAGGAGAUGGGCAAGG-3′

Use the table below to help you answer the following questions. THE CODON TABLE FIRST POSITION SECOND POSITION THIRD POSITION UUU UCU UAU UGU Phenylalanine Tyrosine Cysteine UCC Serine UUC UAC UGC UA UCA UAA Stop UGA Stop Leucine UUG UCG UAG Stop UGG Tryptophan CU CCU CAU CGU Histidine CUC CAC CGC Leucine Proline Arginine CUA CA CAA CGA Glutamine CUG CCG CAG CGG AU ACU AAU AGU Asparagine Serine AUC Isoleucine ACC AAC AGC Threonine AUA ACA AAA AGA Lysine Arginine AUG Methionine Start ACG AAG AGG GUU GCU GAU GGU Aspartate GUC GCC GAC GGC Valine Alanine Glycine GUA GCA GAA GGA Glutamate GUG GCG GAG GGG Original DNA DNA TACGCTAT GAG C Protein Methionine-Arginine-Tyrosine-Serine Mutation #3 DNA TACGCTATCGAGC How would describe this mutation? (choose ALL that apply) Substitution Deletion Frameshift Silent Insertion