Answered: What is “washing” in immunology? What…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Related questions

Question

a) What is “washing” in immunology? What types of interactions does
washing interfere with? What molecules are used for effective
“washing” in the immunology lab?
b) Blocking is also a common feature of immunological assays. Why is
blocking required?
i. A student forgot to block their Western blot membrane before
adding the secondary antibody. They continue and block the
membrane after the secondary antibody step, assuming it
doesn’t matter which order the steps are completed in. How
would this affect the results seen?
ii. A student blocked their ELISA plate with 50 microlitres of
blocking buffer, although their coating, binding and detection
steps were at 100 microlitres. How would this affect the results
seen?

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