Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN: 9780134580999
Author: Elaine N. Marieb, Katja N. Hoehn
Publisher: PEARSON
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A researcher is following an
immunohistochemistry protocol. Before blocking the tissue section, the researcher
incubates the slide in citrate buffer at 100°C for 10 minutes.
a) What is the name of this step?
b) Explain why this step is sometimes required before blocking and incubation with the primary antibody.
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Transcribed Image Text:A researcher is following an immunohistochemistry protocol. Before blocking the tissue section, the researcher incubates the slide in citrate buffer at 100°C for 10 minutes. a) What is the name of this step? b) Explain why this step is sometimes required before blocking and incubation with the primary antibody.
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