Compared to an RNA Vaccine what additional steps does the information in DNA vaccine need to undergo before an antigenic protien is produced?

In immunofluorescence experiments, what is the role of the primary and secondary antibodies? (select all that applies) Group of answer choices The primary antibody binds the protein of interest whereas the secondary antibody binds the primary The primary and secondary antibodies can be interchangeable The secondary antibody is labeled with a fluorophore and is therefore responsible for generating the signal The secondary antibody is less important and therefore the experiment can be performed without it

How do mRNA vaccine work?

1) SDS PAGE gels can be probed with NBT/BCIP to perform an immunoblot true/ false 2) the direction of migration of protiens through a matrix is due to most proteins in natur having a net negative charge  true/false 3)  PBST  is a buffer that is used for blocking the membrane during immunoblotting. true/false 4) the buffer used during for transferring proteins to nitrocellulose methanol. true/false 7) The size of the pores of a polyacrylamide gel dcreases as concentrations of polyacrylamide increases. True or false 10) The secondary antibody used in laboratory for western blotting is conjugated to which enzyme  A) Alkaline decarboxylase B) GAPDH C) Alkaline Phosphatase D) Horseeradish peroxide 13) In protien electrophroresis, what reagent present in the sample buffer is used to eliminate difference in the charge densities of protein A) SDS B) 2- mercaptoethanol C) Tris-HCL pH6.8 D) Tris- HCL PH 8.8