What is transformation efficiency and how is it calculated?

Q: Name one common methods in DNA/RNA extraction. What is the Difference between DNA Purification and…

A: The process of “DNA extraction” is way of isolating and purifying DNA. There are many ways to…

Q: Calculate the melting temperature of this primer and estimate the annealing temperature of this…

A: Introduction PCR (polymerase Chain Reaction) is a molecular technique by which multiple copies of…

Q: what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?

A:

Q: What are the applications of transformation?

A: In genetics and molecular biology, transformation is genetic alteration of a cell. It results from…

Q: what is a replicase enzyme (protein)?

A: Replicase is an enzyme that has the capability to replicate nucleic acids. Enzymes are usually…

Q: What is a poor transformation efficiency?

A: Transformation is a natural phenomenon mostly observe in many bacteria. It is dependent on the…

Q: What is the meaning of DNA clone?

A: Deoxyribonucleic acid (DNA) is a molecule composed of two polynucleotide chains that coil around…

Q: What is the point of hydrolyzing RNA before doing biochemical tests?

A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.

Q: What is the difference between ORF and CDS?

A: ORF (open reading frame) is the portion of a reading frame that has ability to be translated. CDS…

Q: What Is a DNA Library?

A: For constructing DNA libraries, the DNA is processed using restriction enzymes. The components of…

Q: In preparation for transformation, why do you think we used calcium chloride and magnesium chloride…

A: We often use calcium chloride and magnesium chloride to make cell competent. As DNA is hydrophilic…

Q: What is the function of conjugation reactions in the biotransformation process?

A: Biotransformation refers to reactions that modify a specific chemical compound (both endogenous and…

Q: What is the difference between positive and negative supercoiling?

A: The DNA (Deoxyribonucleic acid) is the genetic material that is inherited by offspring over…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: What do you mean by restriction fragments?

A: Recombinant DNA technology is the process of combining two or more DNA fragments from a different…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: What is the hybridization and synthesis process and why it is needed?

A: In the cell, there are several processes, which are required for survival and biochemical functions.…

Q: What does SDS–PAGE stand for? What is the benefit of doing SDS– PAGE?

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: What is the formula for transformation efficiency?

A: Transformation: Transformation is a process of delivering a genetic element inside a bacterial cell.…

Q: Briefly discuss the applications of k-mer analysis.

A: k-mer analysis is used in the field of bioinformatics, computational genomics, and sequence…

Q: What different between the generated amplification product and the primer sequences in the CDS…

A: An increase in the number of copies of a gene in a genome is referred to as gene amplification. In…

Q: What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each…

A: The DNA (deoxyribonucleic acid) is the hereditary nucleic acid sequence that contains the genetic…

Q: What is transformation? How did Avery and his colleagues demonstrate that the transforming principle…

A: In some species of bacteria transformation is naturally occurring process. Transformation is used as…

Q: Which of the following is defined as transformation?

A: Which of the following is defined as transformation?   Answer. Option (C) . Free-floating DNA is…

Q: why are 2D NMR spectra symmetrical

A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…

Q: What are the three main steps in procedures of transformation?

A: Bacterial transformation is a horizontal gene transfer wherein certain bacteria called competent…

Q: Dichotomous key?

A: The dichotomous key is an important scientific tool, used to identify different living things, based…

Q: In a protocol for DNA sample preparation for agarose gel electrophoresis, what volume of 4X loading…

A: Electrophoresis  It is a biochemical technique to separate charged molecules under the influence of…

Q: What increases transformation efficiency?

A: Transformation efficiency refers to the efficiency by which a cell can easily take up a…

Q: Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA…

A: A protein fragment that is obtained when the DNA polymerase I of E.coli is enzymatically cleaved…

Q: What are biochemical characterisation of transforming principle?

A: Frederick Griffith conduct a series of experiments with bacteria streptococus pneumoniae (or…

Q: What is the purpose of a standard curve for a colorimetric enzyme assay?

A: Enzyme assays are mostly used to determine the vitamin status in the body. The activity of…

Q: What is Kozak sequence ? who invented this ?

A: Introduction  The process of transcribing the mRNA into protein is called Translation. There are 3…

Q: What does A230 indicate, in assessing extracted DNA quality?

A: Option C  Extraction contaminants

Q: When is the transformation factor used?

A: According to Griffith's experiments, based on the bacterial transformation through the Streptococcus…

Q: Who revealed biochemical nature of the transforming principle?how was it done?

A: The genetic material refers to the substance present in mitochondria, cytoplasm, and nucleus. It…

Q: What is the purpose of the dideoxyribonucleoside triphosphates in the dideoxy-sequencing reaction?

A: DNA sequencing is carried out using various methods. The majorly used method of DNA sequencing is…

Q: What is the purpose of hydrolyzing the RNA before conducting biochemical tests?

A: Introduction: RNA is a type of nucleic acids that is a genetic material in some viruses.

Q: Contrast the contributions made to an understanding of transformation by Griffith and by Avery and…

A: Microorganisms or microbes have various kinds of interaction with other living things. They exhibit…

Q: What does it mean to hydrolyze RNA before doing biochemical tests?

A: The nucleic ribose acid is RNA. It is a nucleic acid type. There are three kinds of RNA: reran,…

Q: What factors could explain a transformation efficiency?

A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…

Q: What is reductive dechlorination and why is it environmentallyrelevant?

A: Chlorination refers to the process of the addition of chlorine into the water for the disinfecting…

Q: What is the primary disadvantage of Sanger sequencing?

A: Sanger sequencing the target DNA sequence is determined by copying it into the fragments of…

Q: What does A280 indicate, in assessing extracted DNA quality?

A: The correct answer is aromatic amino acids.

Q: what is isosterism and bioisosterism? how does this concept help in biotransformation of different…

A: The term Pharmacokinetics is used to refers to the measurement of the fate of a pharmacological…

Q: What is the functional significance of sticky ends in a cloningexperiment?What type of bonding makes…

A: Answer- Sticky ends are generated after restriction endonuclease is applied on a DNA on the…

Q: What is natural transformation in biology?

A: In biology transformation is the process of genetic alteration of a cell. In this process an…

Q: What is deadenylation-independent decay? What is the role of deadenylation-independent decay ?

A: Degradation of messenger RNAs (mRNAs) plays a vital role in the modulation of expression of gene and…

Q: How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification…

A: Protein purification is a process of isolation of specific protein from the complex protein mixture…