Q: What is the functional difference between regular PCR and qPCCR?
A: PCR stands for a polymerase chain reaction. As the name suggests that polymerase chain reaction…
Q: Outline the DNA extraction steps?
A: The extraction of DNA is an important initial step in the experiments of recombinant DNA technology.…
Q: What benefits are there when using a DNA microarray over a genetic marker such as a STR?
A: The term genetic refers to the DNA molecule present in the nucleus of cells. DNA or deoxyribonucleic…
Q: Describe the technology behind identifying, synthesizing,sequencing, and amplifying DNA.
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: explain the uses of the polymerase chain reaction, gel electrophoresis, and DNA probes, and how they…
A: The application of the polymerase chain reaction, gel electrophoresis, and DNA probes,
Q: In rRT–PCR, why do need to convert RNA to DNA first? Why can’t you amplify the RNA molecule…
A: Taq polymerase does not work on RNA samples, so PCR cannot be used to directly amplify RNA…
Q: How does a DNA microarray work?
A: Gene microarray technology enables to deposit many different DNA sequences on a small glass slide,…
Q: How do we convert RNA to DNA in RT-PCR?
A: Introduction:- The hereditary ingredient in the cell is genetic material. It contains all of an…
Q: What is meant by “proofreading” with respect to DNA polymerase?
A: DNA polymerase belongs to the member of the family of enzymes that play the role in catalyzing the…
Q: How does gel electrophoresis separate different-sized DNA fragments?
A: Gel electrophoresis is a molecular biology technique used to separate macromolecules like DNA, RNA…
Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…
A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…
Q: What are the key differences between DNA microarrays and protein microarrays, and how they are used…
A: Introduction: A laboratory method used to recognize the expression of thousands of genes…
Q: CTAB method is usually used for extracting DNA from eukaryotic samples particularly plant samples.…
A: The steps involved in extraction of DNA are: a. The cells are disrupted to expose the DNA within,…
Q: What type of probe is used for real-time PCR? Explain howthe level of fluorescence correlates with…
A: In the conventional amplification technique of PCR (polymerase chain reaction), the amplified…
Q: Outline the method of in situ hybridization.
A: In molecular biology, hybridization is defined as a technique in which RNA or single-stranded DNA…
Q: How to quantify isolated of DNA samples through spectrometer ?
A: Quantitation of nucleic acids is usually done in molecular biology to estimate the mean…
Q: What are possible mistakes that can cause the DNA to be contaminated during isolation, purification…
A: Various techniques are used to extract different types of DNA. Cells are broken open using a…
Q: EXPLAIN DNA microarrays: Types, Applications and their future
A: DNA microarray is also known as DNA biochip. It is a collection of microscopic DNA spots that are…
Q: What is linear amplification PCR?
A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…
Q: Describe a high-throughput, automated method bywhich millions of DNA templates may be…
A: The process of determining the exact order in which the sequence of the nucleotides are present in…
Q: What are the advantages of Next Generation Sequencing?
A: DNA sequencing is a biochemical method for determining the order of nucleotide bases, A, G, C, and…
Q: Why is the use of a heat-stable DNA polymerase important to the success of PCR?
A: The polymerase chain reaction (PCR) is a method used to amplify the deoxyribonucleic acid (DNA) of…
Q: What are the advantages of doing DNA sequencing?
A:
Q: What is the mechanism of separation of large DNA fragments in pulsed field gel electrophoresis.
A: Pulse Field Gel Electrophoresis is a powerful genotyping technique used for the separation of large…
Q: Discuss the principles , uses, advantages and disadvantages of illumina sequencing method
A: DNA copies obtained from PCR is subjected for gene sequencing through different gene sequencing…
Q: What are the different types of PCR and their product concepts? Site and explain the comparison…
A: Introduction :- Polymerase Chain Reaction (PCR) is a molecular biology technique for making multiple…
Q: What are the major differences between dna probe and Primer
A: DNA as a genetic material in all the living organisms.
Q: What are the advantages and disadvantages of microarraytechnology compared to sequencing PCR…
A: Gene expression studies: In gene expression studies, RNA is isolated from the cell, and cDNAs are…
Q: Explain the limitation of DNA microarrays ?
A: DNA microarray is the molecular laboratory technique that helps in studying and measuring the…
Q: What is DNA microarray used for?
A: It is a laboratory tool used to detect the expression of thousands of genes at the same time. The…
Q: Gel Electrophoresis Separates DNAFragments According to which thing?
A: Electrophoresis is a technique used for separating the components of a mixture of charged molecules…
Q: What is a good transformation efficiency?
A: Transformation is a common phenomenon in microbes like bacteria and results in genetic alteration of…
Q: Why do you think the DNA is stored cold with the InstaGene matrix after boiling the samples and…
A: The polymerase chain reaction (PCR) is used to replicate a specific portion of DNA millions of…
Q: What is the major difference between the strategies of map based sequencing and shotgun sequencing?
A: DNA sequencing is a process in which sequence(order) of nucleotides in DNA is determined. It…
Q: How does a DNA microarray work amd How do you make a DNA microarray?
A: DNA microarray is a collection of DNA sequences attached to a solid surface such as a glass slide…
Q: Prepare an 35 µL PCR reaction mixture and set thermal conditions for gene amplification?
A: PCR or the polymerization chain reaction is the molecular biology technique used to make multiple…
Q: What factors could explain a transformation efficiency?
A: Transformation efficiency is the efficiency with which a bacterial cell can take up the…
Q: How can we use software to identify restriction-enzyme cutting sites in sequenced DNA ?
A: Restriction analysis is the process of identifying restriction mapping sites in DNA sequences by…
Q: What determines the size (length) of the primary PCR product? What might a successful gel check of…
A: PCR stands for polymerase chain reaction. It is used to amplify the specific region of gene of…
Q: Describe several applications of DNA profiling and microarrayanalysis.
A: Deoxy ribonucleic acid (DNA) is the genetic material of most organisms that carry coded genetic…
Q: Describe several methods commonly used for the sequencing of DNA
A: DNA sequencing refers to the sequencing of the nucleotides base pairs in the genome of an organism.…
Q: Name the five key tools for accomplishing the tasks of recombinant DNA technology.also mention the…
A: Recombinant DNA technology is a process of joining of DNA molecules of two different species. This…
what are advantages and disadvantages of using STRmix software in degraded DNA and DNA mixtures?
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- What are the advantages and disadvantages of microarraytechnology compared to sequencing PCR products?Discuss the principles , uses, advantages and disadvantages of illumina sequencing methodPlease briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.
- Gel electrophoresis can be used for all of the following purposes except A) Determining the function of a gene product B Separating DNA (c) Assisting with constructing a restriction map (D) Determining the size of a DNA fragmentHow can site-specific recombination be used in recombinant DNA technology? Explain with an exampleProvide detailed explanation of the following super-resolution techniques: GSD, RESOLFT, SPDM, PAINT or DNA-PAINT.
- CTAB method is usually used for extracting DNA from eukaryotic samplesparticularly plant samples. What does CTAB means? And why is it used as a common buffer solution in DNA extraction protocol?what are the advantages and disadvantages of Gel electrophoresis in DNA analysisWhat is DNA microarray used for?