How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification techniques?

Q: If the target protein is 0.1% of the total protein in the original mixture, a three-step…

A: Purification is a process by which impurities are removed from a sample and desired component is…

Q: Elaborate on three (3) lysis methods used to disrupt bacterial cells.

A: Cell lysis or cell disturbance is a strategy wherein the external limit or cell layer is separated…

Q: why would an EndA+ strain potentially be an issue for DNA Purification if the optional wash step…

A: DNA purification is the process of isolating DNA from a biological sample by removing contaminants…

Q: In standard agarose gels used for the analysis of DNA, linear DNA fragments are separated on the…

A: Agarose gel electrophoresis is the most effective, easy, and efficient way to purify, separate,…

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the…

A: Isoelectric focusing electrophoresis is a technique which is used for separation of amphoteric…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: What types of homogenization techniques are available for solubilizing a protein?

A: In the process of slating out, the solubility of the protein reduces due to the highly ionic salt…

Q: What is the structure of the gel stain being used? There are several other dyes that can bind DNA…

A: Gel staining is very important visualization and detection procedure that follows protein…

Q: Describe the common strategy (steps) for protein sequencing, starting with a biological sample…

A: Proteins are one of the 4 major biomacromolecules. Proteins are the most abundant of the 4…

Q: The following list of proteins are found in a mixture and are to be separated by 2D gel…

A: In 2D gel electrophoresis, is a 2 step-based separation of protein.  In the first dimension, the…

Q: Which of the following gels is the preferred choice for separation of DNA fragments?  Agarose…

A: There are several techniques which are used in recombinant DNA technology. In a recombinant DNA…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: Describe in detail the preliminary purification method which you will use to partially purify a very…

A: Proteins are polymers made by combining amino acids as the monomeric units. Amino acids are combined…

Q: Describe the process used in purification of protein

A: Proteins are larger biomolecules that are made up of a combination of 20 different amino acids, and…

Q: What additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How…

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: How can the foreign protein be modified to facilitate its purification?

A: Protein purification is a progression of cycles proposed to confine one or a couple of proteins from…

Q: You are provided with cultures of E. coli cells that contain the plasmid pUC118. Each student should…

A: Hi, Thanks For Your Question. Answer V: RESUSPENSION Explanation : Solution One : Divalent Cations…

Q: You have an extract of BL21(DE3) cells containing a soluble, 75 kDa recombinant protein that has a…

A: Chromatography is an is a technique used to separate or purify compounds from the mixture of…

Q: Explain the Doyle and Doyle (1990) method of extraction. Identify which step removes specific…

A: Introduction: Since Friedrich Miescher's original DNA extraction in 1869, scientists have made…

Q: What protein purification methods can be used to ensure that the resulting protein is soluble ?

A: The method used for protein purification that can be used to ensure that the resulting protein is…

Q: How can molecular structural analysis be accomplished with electrospray ionization mass…

A: Molecular structural analysis involves determining the arrangement and properties of atoms within a…

Q: why are 2D NMR spectra symmetrical

A: 2D NMR spectra is used to determine the structure of a molecule. It is used for molecules that are…

Q: What are two ways to denature a protein and why do these methods work?

A: Given:  Two ways to denature a protein.

Q: What is the isoelectric point of this fragment?    DHVTHLRKMG

A: Here the given peptide is: DHVTHLRKMG…

Q: What type of probes must be used for super-resolutionmicroscopy and why?

A: The super-resolution microscopy involves a series of techniques that allow high-resolution images.…

Q: If we have different protein samples. How would we analyze them using SDS- PAGE and 2-D gel…

A: Purification of proteins is a critical step in gaining a full understanding of the protein's…

Q: What is used to identify the size of a DNA molecule during gel electrophoresis?

A:

Q: What are some advantages of sequencing peptides by mass spectrometry rather than by Edman…

A: Edman degradation:  It is a three-stage reaction that tags and removes the N-terminal residue of a…

Q: Can native gel electrophoresis of protiens be used to determine protein molecular weight? Explain.

A: Electrophoresis is the motion of dispersed particles relative to a fluid under the influence of a…

Q: What is the purpose of a standard curve for a colorimetric enzyme assay?

A: Enzyme assays are mostly used to determine the vitamin status in the body. The activity of…

Q: (3) What is a protein database? Give examples (and links) of some protein database

A: Bioinformatics is described as the application of tools of computation as well as the analysis to…

Q: The following image is of an agarose gel. If DNA samples were loaded to this gel and the…

A: Electrophoresis is a method that uses an electric field to separate macromolecules in a fluid or gel…