How is PAGE (Polyacrylamide Gel Electrophoresis) different from other protein  purification techniques?

Q: Elaborate on three (3) lysis methods used to disrupt bacterial cells.

A: Cell lysis or cell disturbance is a strategy wherein the external limit or cell layer is separated…

Q: why would an EndA+ strain potentially be an issue for DNA Purification if the optional wash step…

A: DNA purification is the process of isolating DNA from a biological sample by removing contaminants…

Q: What is the point of hydrolyzing RNA before doing biochemical tests?

A: Asked: The reason of hydrolyzing the RNA before doing biochemical tests.

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…

Q: What is the importance of the 6.25 multiplication factor for protein analysis (Kjeldahl methods)?

A: The Kjeldahl method for protein measurement was designed to be used for the measurement of nitrogen…

Q: Both protein and DNA are run together in an isoelectric focusing (IEF) electrophoresis using the…

A: Isoelectric focusing electrophoresis is a technique which is used for separation of amphoteric…

Q: How do you verify amino acid sequences with proteins and species with BLAST?

A: Bioinformatics is an interdisciplinary field of study which has diverse application in the…

Q: Why should there be 3 banding techniques (G, Q, and R)? Aside from their differences in appearance…

A: Banding techniques are utilized to understand the staining effects in the chromosomes so that the…

Q: What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: How does SYBR green work in DNA imaging and why does the uncut plasmid run faster than the cut…

A: DNA imaging can analyze gene expression levels by detecting the presence and quantity of specific…

Q: List all the components and explain their purpose in the reaction mixture used for the dideoxy DNA…

A: The method of evaluating the sequence of nucleotides (As, Ts, Cs, and Gs) in a portion of DNA is DNA…

Q: You have an aqueous solution containing: Alaine (a mono-amino, monocarboxylic acid) Fructose (a…

A: An assay can be defined as an analytic procedure that is used in laboratory medicine, mining for…

Q: What is the structure of the gel stain being used? There are several other dyes that can bind DNA…

A: Gel staining is very important visualization and detection procedure that follows protein…

Q: rationale for column-based DNA extraction and purification.

A: Introduction DNA extraction is a technique that involves methods and procedures to isolate DNA from…

Q: Describe the common strategy (steps) for protein sequencing, starting with a biological sample…

A: Proteins are one of the 4 major biomacromolecules. Proteins are the most abundant of the 4…

Q: what type of gel(in terms of material) can be more suitable for the electrophoresis of 500-1000bp…

A: DNA tests are stacked into spaces toward one side of a gel, and an electric current is applied to…

Q: Describe the two general types of protein microarrays. What aretheir possible applications?

A: A protein microarray is also known as the protein chip, is the high throughput method that is used…

Q: What is oligonucleotide microarray?

A: An array is an orderly arrangement of samples where matching of known and unknown DNA samples is…

Q: What do you mean by fluorescent-labeled probes?

A: Fluorescent marking is the interaction of covalently binding fluorescent colors to biomolecules,…

Q: What additive in the agarose gel solution allows the DNA to fluoresce (glow) under UV light? How…

A: Electrophoresis is the migration of charged particles under the influence of an electric current.…

Q: You are provided with cultures of E. coli cells that contain the plasmid pUC118. Each student should…

A: Hi, Thanks For Your Question. Answer V: RESUSPENSION Explanation : Solution One : Divalent Cations…

Q: You have an extract of BL21(DE3) cells containing a soluble, 75 kDa recombinant protein that has a…

A: Chromatography is an is a technique used to separate or purify compounds from the mixture of…

Q: Explain the Doyle and Doyle (1990) method of extraction. Identify which step removes specific…

A: Introduction: Since Friedrich Miescher's original DNA extraction in 1869, scientists have made…

Q: How can molecular structural analysis be accomplished with electrospray ionization mass…

A: Molecular structural analysis involves determining the arrangement and properties of atoms within a…

Q: What is the mechanism of separation of large DNA fragments in pulsed field gel electrophoresis.

A: Pulse Field Gel Electrophoresis is a powerful genotyping technique used for the separation of large…

Q: What are two ways to denature a protein and why do these methods work?

A: Given:  Two ways to denature a protein.

Q: What is the isoelectric point of this fragment?    DHVTHLRKMG

A: Here the given peptide is: DHVTHLRKMG…

Q: You start with 100 units of protein activity and 100 grams of total protein. After the first…

A: Protein purification is performed to purify a target protein from a mixture of proteins. Fold…

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: The chain elongating inhibitors of the DNA polymerase which are required for the Sanger method of…

Q: What is used to identify the size of a DNA molecule during gel electrophoresis?

A:

Q: A bioactive linear peptide was detected in amphibian toxıns. The peptide was found to be made up of…

A: Given; the amphibian toxin has only 10 amino acids out of which 2 are cysteine (nonpolar)and 2 are…

Q: What are some advantages of sequencing peptides by mass spectrometry rather than by Edman…

A: Edman degradation:  It is a three-stage reaction that tags and removes the N-terminal residue of a…

Q: How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used?…

A: The question is based upon the concept of molarity (number of moles of a compound per litre of…

Q: 5- Which statement is not true for agarose gel electrophoresis? a) DNA fragments are separated based…

A: The technique which is used for the separation of DNA fragments is called agarose gel…

Q: (3) What is a protein database? Give examples (and links) of some protein database

A: Bioinformatics is described as the application of tools of computation as well as the analysis to…

Q: Is a faded gel matrix in a DNA ladder in agarose gel electrophoresis still useable for analysis or…

A: Agarose gel electrophoresis is a technique to separate different fragments of DNA on the basis of…