What is the role of Agrobacterium tumefaciens in the production of transgenic plants?a. Genes from A. tumefaciens are inserted into plant DNA to give the plant different traits.b. Transgenic plants have been given resistance to the pest A. tumefaciens.c. A. tumefaciens is used as a vector to move genes into plant cells.d. Plant genes are incorporated into the genome of Agrobacterium tumefaciens

What is the role of Agrobacterium tumefaciens in the production of transgenic plants?a. Genes from A. tumefaciens are inserted into plant DNA to give the plant different traits.b. Transgenic plants have been given resistance to the pest A. tumefaciens.c. A. tumefaciens is used as a vector to move genes into plant cells.d. Plant genes are incorporated into the genome of Agrobacterium tumefaciens

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter10: Biotechnology

Section: Chapter Questions

Problem 6RQ: What is the role of Agrobacterium tumefaciens in the production of transgenic plants? a. Genes from...

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What potential problems must be considered in creating a transgenic bacterium with the human insulin gene isolated from genomic DNA to produce insulin? a. The genetic code of bacteria is significantly different from the genetic code of humans. b. The bacterial cell will be unable to posttranslationally process the insulin peptide sequence. c. There is no way to get the bacterium to transcribe high levels of a human gene. d. Both a and b present problems.
A. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa? C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?
Which of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production An SNP is found associated with individuals who have diabetes. Which statement is true? A. All individuals with the SNP will have diabetes. B. The SNP codes for a faulty protein that causes the individual to have diabetes. C. To identify the SNP in more individuals, they will need to have their genome sequenced. D. The SNP may be linked to a gene that contributes to diabetes.
Which of the following would be considered a transgenic organism? A. a human whose human blood-clotting gene was silenced B. a fern grown in cell culture from a single fern root cell C. a rat with rabbit hemoglobin genes
Which is the BEST technique to use to answer this question? You wish to determine whether a point mutation is present in the sunscreen gene. a. Westem blot. b. Northern blot . c. Sanger DNA sequencing d. In situ hybridization. Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo. a. Immunofluorescencez b. Western blot . c. RT-PCR. d. Northern blot. e. in situ hybridization.
What is a transgenic organism? a. an organism that has had its entire genome sequenced b. an organism that has genes from another species c. an organism that does not use DNA to specify proteins d. an organism with multiple copies of the same gene e. an organism that has been DNA profiled
Which of the following is an outcome that could be produced by the application of the biotechnology as described? A. using PCR to modify a crop plant for higher yields B. introducing a recombinant DNA plasmid into a cell to trigger RNAi C. identifying SNPs to match birth parents to an adopted child D. using RT-PCR to generate copies of a gene to be expressed for insulin production
What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blot
When A. tumefaciens is used to make a transgenic plant, agene of interest is inserted into a _________, which is firsttransformed into __________. The __________ is then transferredto a plant.a. viral vector, E. coli, gene of interestb. T-DNA vector, A. tumefaciens, T DNA carrying the gene ofinterestc. T-DNA vector, A. tumefaciens, gene of interest aloned. T-DNA vector, E. coli, gene of interest alone
What is a biomarker? a. the color coding of different genes b. a protein that is uniquely produced in a diseased state c. a molecule in the genome or proteome d. a marker that is genetically inherited
Part A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprinting
Many concerns have been raised about the safety of GMOs. These are all concerns that have been raised EXCEPT A. Genes that offer herbicide resistance will be passed on to weedy species making "superweeds". b. GM crops will cross with non-GM crops and move the recombinant genes into crops where farmers don't want them. c. Genes added to crop plants can negatively affect the health of people who eat the crops. d. Genetically modified crops increase the use of fossil fuels and therefore increase the production of carbon dioxide.