Design a DNA microarray experiment that measures the difference in gene expression between normal colon cells and cells from a colon tumor.
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Design a DNA microarray experiment that measures the difference in gene expression between normal colon cells and cells from a colon tumor.
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- When doing the virtual DNA microarray analysis, which color (red, green or yellow) would the spot be if that gene was inappropriately inactivated in the cancer cell? When doing the virtual DNA microarray analysis, which color (red, green or yellow) indicated that the cancer cell had an activated a gene that is not normally as active in the non-cancerous version of the cell?Using a flow diagram, elaborate on how you would generate a recombinant plasmid that can be used for the expression of a therapeutic insulin.Recombinant bacteria can produce hormones that are normally produced in humans. Briefly describe how this is accomplished.
- Which vector is used to replace the defective gene in gene therapy?Describe the steps of the chromatin immunoprecipitation assay. What are some of the scientific facts that can be learned through a chromatin immunoprecipitation assay?Bacteria can be used to produce human growth hormone (HGH - a peptide/protein) through genetic engineering. The human gene for HGH is inserted into a plasmid, which is then taken up by a bacterial cell, which divides and multiplies into a clone of cells, all of which contain the plasmid with the HGH gene. The bacteria express the HGH gene, producing HGH which can be harvested and used for treatment of humans. (See figure below) Which of the following statements is NOT true about this process? bacterium Vector, such as a DNA containing the gene of plasmid, isolated it from a different species is Gene encoding protein for pest resistance is inserted into plant cells ©2019 Pearson Education, Inc chromosome recombinant DNA (plasmid) transformed bacterium Create and harvest copies of a gene with either of two goals in mind. Gene encoding degradative enzyme to clean up toxdo waste is inserted into bacterial cells ved by an enzyme into gene of interest The desired gene is selected and…
- Outline a PCR-based approach to screen for colonies transformed with recombinant expression plasmid that can be used for protein expression.Given these options, which best describes the Cre-Lox system A) can be applied to control the expression of any gene desired by researchers. B) can only be used to control expression of genes encoding fluorescent proteins. C) is interesting in theory, but cannot be applied practically in research D) Only allows us to create glowing mice for the pet industry.From your knowledge about DNA microarray, answer the following: If the expression microarray experiment was done with a normal sample and a suspected sample, after reading the color pattern resulted from the experiment it was recorded that “gene A22” is expressed in the suspected sample. The gene A22 is clinically linked to colon cancer. Answer the following: What is the expected color of the spot on the microarray which represents this gene? What is your interpretation of the suspected sample; is it a cancer sample or not and explain why?
- How are expression vectors useful?a) To produce protein productsb) Used for genomic librariesc) Used for chromosome synthesisd) Used for finger printingFrom microarray analysis how do we know what genes are being expressed in a specific tissue?In the experiment summarized below, scientists were examining the presence of specific sequences in individuals with age. In this experiment they extracted DNA from lymphocytes of various aged individuals and measured the length of a TTAGGG (in kb) repeat they found in their genomic DNA (Left Panel). In the right panel, the scientists measured the length of the same repeats in individuals with lymphocyte failure (red dots most severely effected) that have a mutation in a critical enzyme. Answer the following questions in 2-3 sentences each. A. What is the name of the specific sequence the scientists are measuring in the experiment shown below. B. For the individuals with lymphocyte pathology in the right panel, which gene is likely defective that causes the data shown? C. Explain why the length of the repeat sequence decreases with age.