Imagine a cell that truly has a Gram-positive style of cell wall. Which of the following staining errors would cause the cell to give a Gram-negative reaction? Assume that only 1 error is made at a time, but more than 1 type of error MAY be able interfere with the Gram stain. Mark all that are correct. 1. Forgetting to apply the crystal violet 2. Forgetting to apply the iodine 3. Forgetting to apply the alcohol 4. Leaving the alcohol on for 60 seconds (too long) 5. Forgetting to apply the safranin

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Imagine a cell that truly has a Gram-positive style of cell wall. Which of the following staining errors would cause the cell to give a Gram-negative reaction? Assume that only 1 error is made at a time, but more than 1 type of error MAY be able interfere with the Gram stain.

Mark all that are correct.

1. Forgetting to apply the crystal violet
2. Forgetting to apply the iodine
3. Forgetting to apply the alcohol
4. Leaving the alcohol on for 60 seconds (too long)
5. Forgetting to apply the safranin
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Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents

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