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- 5. Show the separation pattern of the following DNA molecules on an agarose gel electrophoresis. 5 kbp 5 kbp 5 kb 5 kbp ----What enzyme proofreads the DNA molecule? Group of answer choices DNA gyrase DNA polymerase I DNA helicase DNA polymerase IIISelect all that are true about DNA gel electrophoresis O This technique can be used to separate DNA based on the size of the fragment O Since DNA is positively charged it will travel to the negative electrode O After a gel is run, the larger fragments end up being further away from the well The rate at which DNA fragments travel is logarithmically and is inversely proportional to its size
- How do I translate the DNA sequence below: 5'-ATGGCCTGGCATTCA-3' 3'-TACCGGACCGTAAGT-5'In a standard procedire, when writing and reading base sequences for nucleic acids (both DNA and RNAs) always to specify base sequence in 5' > 3' direction unless otherwise directed 1. From the base sequence 5' A-T-G-C-C-A 3' in a DNA template strand, determine the base sequence in hnRNA synthesized from the DNA template strand 2. From the base sequence 5' T-A-A- C-C-T 3' in a DNA template strand, determine the base sequence in hnRNA synthesized from the DNA template strandOrder the steps required to sequence a region of DNA using dideoxy sequencing. Amplify the region of DNA to be sequenced add a primer, deoxynucleotides, labeled dideoxynucleotides, and DNA polymerase a primer binds to the single-stranded DNA template DNA polymerase extends the primer, incorporating deoxynucleotides a labeled dideoxynucleotide terminates the growing DNA chain gel electrophoresis separates the mixture of DNA fragments by size The DNA sequence is determined denature the double-stranded DNA Answer Bank
- A Sanger product of the sequencing of a template DNA is presented +ddTTP +ddATP +ddCTP +ddGTP Mononucleotide Pentanucleotide Trinucleotide Dinucleotide 11-nucleotide Hexanucleotide Octanucleotide Tetranucleotide 16-nucleotide Decanucleotide Nonanucleotide Heptanucleotide 17-nucleotide 15-nucleotide 13-nucleotide 12-nucleotide 18-nucleotide 20-nucleotide 14-nucleotide 19-nucleotide 21-nucleotide 1. Determine the sequence of template DNA 2. Determine the mRNA sequence from this template DNA 3. Determine the the sequence of protein product derived from that DNAIn the following gel showing stained bands of the Alu insertion sequence, what is the genotype of individual 2? 941 bp 641 bp->>> 1 2 3 4 5 6 Homozygous for the 641 bp sequence that does not contain in the Alu insertion Heterozygous, containing one 941 bp sequence and one 641 bp sequence O Homozygous for the 941 bp sequence containing the Alu insertionRNA Polymerase What enzyme is combining two DNA fragments together? 5' 3' -Template Strands Replication Fork 3' DNA polymerase Okazaki Fragments DNA Ligase Lagging Strand Leading Strand 3' 5' 3' 5' O DNA Helicase DNA Ligase DNA polyermase RNA Polymerase
- Which of the following does the enzyme primase synthesize? DNA primer RNA primer Okazaki fragments phosphodiester linkageTranscribe and translate the following DNA sequence (nontemplate strand): 5’-ATGGCCGGTTATTAAGCA-3’Consider prokaryotes, choose the CORRECT enzyme/keyword for the following function/role: Removes RNA primer and replaces it with newly synthesized DNA. [ Select ]
