What is the basis for the technique called ELISA?
Q: What is indirect immunofluorescence microscopy?
A: BASIC INFORMATION MICROSCOPY It is a field in which the microscope used in order to objects which…
Q: ELISA is used to detect viruses where the key reagent is?
A: Immunoassay is a technique that has the capability to detect even minute quantities of antigen or…
Q: Why do you coat the elisa plate with albumin?
A: ELISA It is enzyme linked immunosorbent assay. It is a common and powerful technique used for…
Q: What are the four steps of an Elisa protocol
A: Enzyme-linked immunosorbent assay (ELISA) is an immunological assay commonly used to measure…
Q: What does ELISA stand for?
A: A very efficient technique used in detecting and capturing a particular protein from a complex…
Q: Which tests would be faster to perform? PCR or an ELISA test?
A: ELISA ELISA is enzyme linked immunosorbent assay. It is based upon antibody-antigen interaction.…
Q: What is Clinical test?
A: A disorder of structure or function in any living organism is called disease. This generally…
Q: How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?
A: Answer: Rapid Strep A Test (Rapid strep test) is a test used to identify the presence of Strep A…
Q: What are the different solid phases that antibody or antigen can bind to, in ELISA?
A: Quantitative immunological techniques that use a solid phase can be defined as solid-phase…
Q: You performed an ELISA and upon looking at your results, you realized you torgot to coat the wells…
A: A key feature of the plate based ELISA is that capture protein that is antibody or antigen can be…
Q: What is DNA barcoding, and what types of cases use this profiling method?
A: DNA barcoding is an instrument to recognize various species. Mitochondrial DNA inheritance ( mtDNA)…
Q: What is the procedure of beta method?
A: Beta-lactamase test is used for the detection of enzyme beta-lactamase. This enzyme provides the…
Q: n not more than 100 words, What antigens/strains are used for the Weil-Felix test, and from what…
A: Well Felix test is an agglutination test for the diagnosis of Rickettsia infection. The Well Felix…
Q: What is the principle involved in the Acrolein test? What is its purpose?
A: Qualitative tests are the chemical or physical tests involved in identification of certain…
Q: what are the important principle in invertase test?
A: To determine enzymatic activity, the amount of substrate remaining or the amount of product created…
Q: ELISA requires the use of blocking solution; what is the purpose of the solution?
A: The enzyme-linked immunosorbent assay captures target antigens (or antibodies) in samples using a…
Q: In the “sandwich” ELISA technique, what is the function of the capture antibody? To link an enzyme…
A: Immunoassay are techniques that have the capability to detect even minute quantities of antigen or…
Q: What are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase…
A: A nasopharyngeal swab(NPS) is a clinical test method which collects test sample from nasal…
Q: What are some ways that the antibody-protein complex can be visualized in ELISA or western blots?
A: Introduction: The theory of antibody/antigen binding is used in ELISA assays. They make it possible…
Q: What other infections or conditions can be detected by an ELISA?
A: The Enzyme-Linked Immunosorbent Assay (ELISA) is a technique used to detect antibodies or infectious…
Q: raw stages of Elisa
A: ELISA stands for enzyme-linked immunosorbent assay. It is an assay technique used for detecting and…
Q: What does the direct ELISA test for?
A: ELISA(enzyme-linked immunosorbent assay) is a biochemical assay to detect the presence of ligand…
Q: Explain indirect and sandwich Elisa.
A: Enzyme linked immunosorbent assay (ELISA) is a wet-lab type analytical assay that used solid phase…
Q: What is the proper way of Modified Allen test?
A: Allen test are the tests used to assess the arterial blood supply of the hands. This test is used…
Q: What is the immunoassay analyzer Machine
A: Immunoassay is a test that is used to measure the concentration of a macromolecule or a small…
Q: What biochemical tests did we use to distinguish staphylococci from streptococci?
A: Staphylococcus and streptococcus both occur in cocci shape and both are gram positive organisms.…
Q: What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is…
A: ELISA is an immunological assay commonly used to measure antibodies, antigens, proteins and…
Q: If you did the multiple-tube technique, list one advantage and one disadvantage of each method of…
A: Water from various sources obtained for routine testing were analyzed by Swedish standard methods…
Q: What is the coliform test when is it used? What is its purpose? How does it function? What are the…
A: All water that is naturally exposed to the atmosphere, such as rivers, lakes, and reservoirs. It is…
Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…
A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…
Q: If you forgot to wash the wells after you added the secondary antibody, what would you expect to see…
A: ELISA stands for enzyme-linked immunosorbent assay. It is a plate-based assay technique designed for…
Q: What is the principle behind Liebermann-Burchard test? And What is the clinical significance of…
A: Liebermann-Burchard test used for estimation of unsaturated steroid (cholesterol) and…
Q: Why do you think it is called indirect ELISA?
A: ELISA is used to detect the antigens or proteins by using specific antibodies against them.…
Q: If you were using the ELISA to look for the presence of antibodies and the sample gave a negative…
A: Biotechnology is a branch of biology, including the use of living organisms to produce products.…
Q: In the plaque assay, what is the precise origin of a single plaque?
A: Answer: Introduction: Virus is an intracellular infectious parasite consist of only one type of…
Q: For what ELISA is used?
A: A wide range of serological tests are available to recognize the antigens or antibodies in the…
Q: In an indirect ELISA what does the secondary antibody do (i.e, what purpose does it serve)?
A: ELISA or Enzyme Linked Immunosorbent Assay is a technique that quantifies and detects soluble…
Q: What is the purpose of the Ames test? How are his− bacteria used in this test?
A: Ames test is also known as Bacterial reverse mutation test. It is a method to test a chemical…
Q: How is a specific probe obtained?
A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…
Q: What is the difference between the total and faecal coliform
A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…
Q: Explain how the competitive ELISA works and what it is used for?
A: Competitive ELISA is a method for determining the presence of antibodies in a sample, such as serum.…
Q: What is the vitro mixture ?
A: All living organisms are a complex entity of a lot of chemical and biological reactions. A…
Q: What about a Katz Test? How is this different or similar to Barthel’s Test?
A: Katz activities of daily living (ADL) test is a gold-standard test used to quantify the functional…
Q: What technique is used to stain bacteria? Who developed this technique?
A: Microorganisms are such small living organisms that are less than 0.1 mm, and can be seen only under…
Q: What is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test?…
A: Modified Furter-Meyer Test is used to detect the presence of alpha-tocopherols.
What is the basis for the technique called ELISA?
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- what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the purpose of each variant in clinical diagnosis?What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?Explain the mechanisms of the three types of ELISA we discussed in lab: 1) direct, 2) indirect, 3) sandwich. Which type of ELISA did we do in lab? What is one advantage of this kind of ELISA?