question 2. To detect trisomy 18 in amniotic fluid cells the most optimal probe type to use is A) M-BCR/ABL probe B) DiGeorge Probe C) Whole chromosome paint probe D) A probe specific for highly repeated alphoid DNA sequences

What is the role of the liquid detergent used in the experiment?  a) It allows the DNA to unwind. b) It aids filtration. c) It helps break down the cell membrane. d) It helps keep the DNA soluble in solution.

Q11) Explain, using the pCR 2.1 vector, what we would see if we try to grow cells on a plate with ampicillin and Xgal in each of these scenarios:a)         transformation not successful (meaning the plasmid did not go inside the bacteria)b)         transformation was successful, but no insert in LacZ gene (plasmid went in the bacteria but there was no inserted DNA in the LacZ gene of the plasmid)c)         transformation and the ligation reactions were successful (got the plasmid in, and the plasmid is carrying extra DNA in the LacZ region, yay)

a) Sam wants to alter his interrupted mating experiment by placing a filter in between the donor and the recipient cells in order to separate the two. Is Sam’s suggestion for this modification to the procedure utilized in interrupted mating experiments valid? Explain.   b) Much to the dismay of the researcher with whom Sam is working, Sam is not only forgetful, but he is also lazy! Rather than devoting the necessary hours to conducting his assigned interrupted mating experiments, Sam has decided that his time would be better spent modifying the F plasmid so that the experiments wouldn’t require quite so much time. The diagram below shows a map of the E. coli F plasmid, similar to that shown in lecture. Sam is determined to “get rid of some of the excess DNA” present in the plasmid. Please keep in mind that the PI in the lab in which Sam works utilizes these F plasmids for interrupted mating experiments between Hfr and F- cells as well as for interrupted mating experiments involving F+…

If you use the pUC18 vector  to clone in the MCS region, predict the following: a) Do bacteria that are blue in color have a cloned insert? b)Do bacteria that are white in color have a cloned insert?  c) If you were to grow these cells on Chloramphenicol (an antibiotic), would the bacteria with the pUC plasmid grow? Why or Why not?

Which of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene from mRNA C) subcloning a bacterial gene from one plasmid to another D) DNA fingerprinting for paternity testing E) DNA sequencing

What was the purpose for doing the colony PCR?

What is SDS and what part does it play in the alkaline lysis method of preparing plasmid DNA from a culture of bacteria?

Part 1. Gene Cloning   1) How is a gene inserted into a plasmid cloning vector? Be specific, but be brief. No more than 2 (non-run-on) sentences please, and do not exceed this space.             2) A geneticist has transformed a vector with an ampicillin resistance gene into E. coli cells and plated those cells on media containing ampicillin.  She comes back 24 hours later and sees only blue colonies on the media. a) How does she know that the cells growing on the media were successfully transformed with the vector?           b) What does it mean that the colonies are blue?