What are the four steps of an Elisa protocol

ELISA is a vital diagnostic tool. Reagents required for this technique include Thermostable DNA polymerase, RNA primers, and antibodies latex beads, antibodies, glass slide, serum Antigen, Antibodies, microtiter plate, and detection substrate. Thermostable DNA polymerase, DNA primers, and nucleotides

What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?

From the xray how can you say that it is the xray of a child rather than a grown man.

You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).

In the more common protocol for immunofluorescence detection of cellular proteins, an investigator uses two antibodies. The first binds specifically to the protein of interest. The second is labeled with fluorochromes for easy visualization, and it binds to the first antibody. In principle, one could simply label the first antibody and skip one step. Why use two successive antibodies?

Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…

What is the effect of not adding the primary antibody to the ELISA reaction?

A MLS supervisor is writing an Standard Operating Procedure (SOP) for running a PC test for Hepatitis B DNA. There are several things she must include in the instructions to run the test.1. On average, how many PCR cycles must be run to see if the test is positive or negative for HBV?2. What would a positive test and a negative test look like?3. Her younger sister wants to know what she did at work today. How should she explain what happens during each step of the PCR cycle.

Briefly describe two limitations of an ELISA