What is the advantage of using the neo gene to disrupt the function of a gene in knockout mice? The neo gene produces an antibiotic that kills unwanted cells The neo gene produces a toxin that inhibits transcription off the target gene The neo gene facilitates homologous recombination The neo gene is the right size for disabling other genes The neo gene provides a selectable marker for finding cells that contain the disabled gene

After sequencing a new genome for the first time, functional protein-coding genes are initially identified by:     BLAST searches that look for viral integration in the host genome     Bioinformatic searches that reveal consensus sequences in expressed genes     Aligning expressed exons sequenced from cDNA using transcriptomics     B and C     A and B Many regions of non-coding eukaryotic DNA previously thought to be "junk" are now known to contain sequence elements important to regulating gene expression. What approach can be used to identify important non-coding regulatory regions when annotating a newly sequenced genome?     comparing cDNA to genomic DNA to validate that the gene is expressed     identifying restriction enzyme recognition sequences in the genome     phylogenetic footprinting to identify conserved non-coding sequences     searching for start/stop codons and splice recognition sites that predict where a gene might be located…

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In gene therapy, why can't a gene be inserted directly into the cell? It was mentioned on this website: https://ghr.nlm.nih.gov/primer/therapy/procedures and many others but there's no explanation for it.

Which of the following mutations would not convert a proto-oncogene into an oncogene?     A translocation that places the gene at a new locus where gene expression is increased.     A mutation in the coding region that makes the protein resisitant to degradation.     Gene duplication.     A mutation in the coding region that makes the protein hyperactive.     A deletion of an enhancer that lies 3’ to the coding region.

Define and give examples of: -true reversions -intragenic site reversion -second site reversion

You are analyzing cells that express a fusion gene encoding a FLAG-tagged protein of interest. Upon amino acid sequence analysis, you find that the FLAG protein sequence aligns to the sequence at the end of the fusion protein sequence you have. Where is the tag sequence located?   Group of answer choices At the 5’ end of the gene and the C-term of your protein of interest At the 5’ end of the gene and the N-term of your protein of interest At the 3’ end of the gene and the C-term of your protein of interest At the 3’ end of the gene and the N-term of your protein of interest

Which construct would you use to make a mouse model ?

Which of the following observations give support to the idea that changes in chromatin structure affect gene activity? nucleosome removal near active genes 0000 position effect variegation formation fo Barr bodies in mammals all of the above

An organism no longer needs to express a particular gene. What is one strategy it might use of the methods discussed in class? Select all that apply. 0.000 de-methylate C bases in the genome acetylate C bases in the genome methylate C bases in the genome acetylate histones de-acetylate histones de-acetylate C bases in the genome