in the cloning vector, what would be the plausible impacts if a mutation at the Ori site renders it non- functional?

Earlier, we described the Lederbergs’experiment, which demonstrated thatmutations are not directed by the environment. But mutagens, which areenvironmental, can lead to mutations. What’s the difference?

What is the purpose of the rat liver extract in this procedure?

How the Mutagenicity can be tested ?

in ecoli, a ligase was deleted from the genome. the cells were then placed in a slighly higher temp. would the ligase deletion matter?

What is the Ames test and how is it carried out? What assumption concerning mutagenicity and carcinogenicity is it based upon?

How can the Ames test distinguish mutagens that cause small insertions /deletions from those that cause base substitutions?

The occurrence of multiple transposons within the genome oforganisms has been suggested as a possible cause of chromosomalrearrangements such as deletions, translocations, and inversions.How could the occurrence of transposons promote these types ofstructural rearrangements?

Can DNA recombinantion be used to insert human genes into human cells; if so, what are the advanatages of inserting the human gene directly into human cells?

E17. Gene mutagenesis is also used to explore the structure and function of proteins. For example, changes can be made to the coding sequence of a gene to determine how alterations in the amino acid sequence affect the function of a protein. Let's suppose that you are interested in the functional importance of a particular glutamic acid (an amino acid) within a protein you are studying. By site-directed mutagenesis, you make mutant proteins in which this glutamic acid codon has been changed to other codons. You then test the encoded mutant proteins for functionality. The results are as follows: Functionality (%) Normal protein 100 Mutant proteins containing Тугosine Phenylalanine 3 Aspartic acid 94 Glycine From these results, what would you conclude about the functional significance of this glutamic acid within the protein?