in the cloning vector, what would be the plausible impacts if a mutation at the Ori site renders it non- functional?

Q: discuss what is mutagenesis and what mechanisms are invloved?

A: Mutagenesis : It is the process by which an organism's deoxyribonucleic acid change , resulting in a…

Q: Describe how the Ames test screens for potential environmental mutagens. Why is it thought that a…

A: Ames test is a biological assay to estimate the ability of a chemical compound to cause mutation.…

Q: During your experiment you analysed only a few of the recombinant clones for the presence of the…

A: A Genomic library consists of the total genomic DNA of an organism. This DNA can be stored inside…

Q: Do you consider TEs to be mutagens? Explain.

A: Mutation is defined as sudden inheritable change that occurs in the DNA sequence. Mutation occurs…

Q: using illustrations describe the structure of a typical cloning vector and discuss the functions of…

A: Vector is a DNA molecule that is used as a carrier or vehicle for carrying foreign genetic material…

Q: Explain the primary mechanisms of each of the threetypes of mutagens

A: Step 1 Mutagenesis is the procedure of causing mutation by a number of physical, biological, or…

Q: Cloning of a gene from the rubber tree can be carried out in bacterial cells. However, for the…

A: Cloning: It refers to the process of creating multiple identical copies of gene-sized pieces of DNA.…

Q: A series of exonuclease deletions were used to study the promoter of the rice hemA gene, giving the…

A: A promoter can be defined as a DNA sequence where proteins bind and initiate transcription of an…

Q: Besides cloning vectors, there are also expression vectors. What is the main differenc between these…

A: cloning vector is a small piece of DNA that can be inserted into organism for cloning purpose.…

Q: You want to clone a eukaryotic gene and express the protein in yeast. The protein typically…

A: Cells are divided into organelles which differ in their protein composition and cells must target…

Q: What is homologous recombination and where/when does it typically take place in an organism.…

A: Homologous recombination is a genetic exchange process that occurs between two similar or identical…

Q: Although a large number of mutagenic chemicals are known,none is known that induces mutations in…

A: Mutation is the process of abrupt changes in the nucleotide sequence or gene in the DNA. Mutation…

Q: Gerald Rubin and Allan Spradling devised a method of introducinga transposon into Drosophila. This…

A: Plasmids are the genetic structures, which can replicate independent of chromosomes. They are the…

Q: Transposon mutagenesis

A: Transposons: These are also known as jumping genes. These are the segments of DNA which can move or…

Q: How can telomerase-related aging be addressed therapeutically?

A: Introduction DNA is packed inside the cell in the form of chromosomes and chromatin. The…

Q: Site directed mutagenesis is used to: 1.determine the critical base per sequences in a Genome 2.…

A: DNA consists of double strands of sequentially arranged nucleotides. The nucleotide sequence present…

Q: Not all inherited traits are determined by nuclear genes (i.e., genes located in the cell nucleus)…

A: The process of producing a genetically identical clone of an animal is called as reproductive…

Q: Discuss the process and principle involved for screening/selection of hosts (last stage of cloning)…

A: Molecular cloning or cloning is a technique to assemble DNA of two different organisms and directing…

Q: In yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Describe…

A: Telomeres, specialized nucleotide sequences located at the ends of chromosomes, play a crucial role…

Q: How many restriction fragment length polymorphisms are there in the humangenome?

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: When disrupting a mouse gene by knockout, why is it desirable to breed mice until offspring…

A: In knockout animal, a targeting vector having a gene of interest is introduced into the cells. The…

Q: Cloning of a eukaryotic gene can be carried out in Escherichia coli. However, for the protein…

A: Introduction Eukaryotes are organisms having well-defined cellular organelles that are specified to…

Q: Discuss the processes of conjugation, transformation (Griffith’s experiment), andtransduction. What…

A: Genetics is a branch of science that deals in the study of genes, heredity, and genetic variation of…

Q: Discuss the role (presence and absence) of a selectable marker (selection) in cloning a recombinant…

A: Recombinant DNA molecule is defined the molecule in which an isolated DNA fragment or gene of…

Q: How are candidate genes that are identified by positional cloning evaluated to determine whether…

A: Gene is a functional unit of heredity. A gene is a sequence of nucleotides in genome that codes for…

Q: Some vectors used in cloning experiments contain bacterial promotersthat are adjacent to unique…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: Identify and briefly describe three of the processes by which deamination of DNA-cytosine residues…

A: Deamination is the removal of an amino group from a molecule. Enzymes that catalyse the reaction…

Q: A person with a rare genetic disease has a sample of her chromosomessubjected to in situ…

A: The word insitu derived from the Latin word that means in place. This suggests that the…

Q: yeast cells, telomerase remains active and maintains telomeres of about 300 base pairs. Propose what…

A: The end areas of chromosomes are called telomeres , are repetitive sequence that protect gene from…

Q: Most retrotransposons include a gene that encodes a transposase enzyme. What does this enzyme do ?

A: Transposable components ar deoxyribonucleic acid sequences which will move regarding within the…

Q: What are the key modifications to the PCR primers, SHFw1 and SHRv1 in order for you to clone the PCR…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: Mutagenesis is a technique in which genetic information of an organism is altered in a stable manner…

A: There are a few important points : Mutation: A sudden and heritable change in the sequence of the…

Q: To cause cancer, proto-genes require blank allele(s) to be mutated and therefore are considered…

A: ANSWER;- To cause cancer, proto-genes require a single allele(s) to be mutated and therefore are…

Q: Suppose a researcher previously cloned gene Y into M13 bacteriophage vector. Gene Y encodes a…

A: In order to solve this problem, first we need to find the amino acid sequence of the wild (original)…

Q: Both transposase and integrase cleave the target DNA at staggeredsites before the TE is inserted.…

A: The transposable elements are DNA (deoxyribonucleic acid) sequences that migrate from one location…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.